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Ovothiol A is the Main Antioxidant in Fish Lens

1
International Tomography Center SB RAS, Institutskaya 3a, 630090 Novosibirsk, Russia
2
Novosibirsk State University, Pirogova 2, 630090 Novosibirsk, Russia
*
Author to whom correspondence should be addressed.
Metabolites 2019, 9(5), 95; https://doi.org/10.3390/metabo9050095
Received: 18 April 2019 / Revised: 8 May 2019 / Accepted: 8 May 2019 / Published: 10 May 2019
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Abstract

Tissue protection from oxidative stress by antioxidants is of vital importance for cellular metabolism. The lens mostly consists of fiber cells lacking nuclei and organelles, having minimal metabolic activity; therefore, the defense of the lens tissue from the oxidative stress strongly relies on metabolites. Protein-free extracts from lenses and gills of freshwater fish, Sander lucioperca and Rutilus rutilus lacustris, were subjected to analysis using high-field 1H NMR spectroscopy and HPLC with optical and high-resolution mass spectrometric detection. It was found that the eye lenses of freshwater fish contain high concentrations of ovothiol A (OSH), i.e., one of the most powerful antioxidants exciting in nature. OSH was identified and quantified in millimolar concentrations. The concentration of OSH in the lens and gills depends on the fish genus and on the season. A possible mechanism of the reactive oxygen species deactivation in fish lenses is discussed. This work is the first to report on the presence of OSH in vertebrates. The presence of ovothiol in the fish tissue implies that it may be a significantly more common antioxidant in freshwater and marine animals than was previously thought.
Keywords: antioxidant; mass spectrometry; freshwater fish; NMR spectroscopy; ovothiol A antioxidant; mass spectrometry; freshwater fish; NMR spectroscopy; ovothiol A
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).

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Yanshole, V.V.; Yanshole, L.V.; Zelentsova, E.A.; Tsentalovich, Y.P. Ovothiol A is the Main Antioxidant in Fish Lens. Metabolites 2019, 9, 95.

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