Postprandial Metabolic Effects of Fiber Mixes Revealed by in vivo Stable Isotope Labeling in Humans
AbstractFood supplementation with a fiber mix of guar gum and chickpea flour represents a promising approach to reduce the risk of type 2 diabetes mellitus (T2DM) by attenuating postprandial glycemia. To investigate the effects on postprandial metabolic fluxes of glucose-derived metabolites in response to this fiber mix, a randomized, cross-over study was designed. Twelve healthy, male subjects consumed three different flatbreads either supplemented with 2% guar gum or 4% guar gum and 15% chickpea flour or without supplementation (control). The flatbreads were enriched with ~2% of 13C-labeled wheat flour. Blood was collected at 16 intervals over a period of 360 min after bread intake and plasma samples were analyzed by GC-MS based metabolite profiling combined with stable isotope-assisted metabolomics. Although metabolite levels of the downstream metabolites of glucose, specifically lactate and alanine, were not altered in response to the fiber mix, supplementation of 4% guar gum was shown to significantly delay and reduce the exogenous formation of these metabolites. Metabolic modeling and computation of appearance rates revealed that the effects induced by the fiber mix were strongest for glucose and attenuated downstream of glucose. Further investigations to explore the potential of fiber mix supplementation to counteract the development of metabolic diseases are warranted. View Full-Text
- Supplementary File 1:
PDF-Document (PDF, 1563 KB)
Share & Cite This Article
Schlicker, L.; Boers, H.M.; Dudek, C.-A.; Zhao, G.; Barua, A.; Trezzi, J.-P.; Meyer-Hermann, M.; Jacobs, D.M.; Hiller, K. Postprandial Metabolic Effects of Fiber Mixes Revealed by in vivo Stable Isotope Labeling in Humans. Metabolites 2019, 9, 91.
Schlicker L, Boers HM, Dudek C-A, Zhao G, Barua A, Trezzi J-P, Meyer-Hermann M, Jacobs DM, Hiller K. Postprandial Metabolic Effects of Fiber Mixes Revealed by in vivo Stable Isotope Labeling in Humans. Metabolites. 2019; 9(5):91.Chicago/Turabian Style
Schlicker, Lisa; Boers, Hanny M.; Dudek, Christian-Alexander; Zhao, Gang; Barua, Arnab; Trezzi, Jean-Pierre; Meyer-Hermann, Michael; Jacobs, Doris M.; Hiller, Karsten. 2019. "Postprandial Metabolic Effects of Fiber Mixes Revealed by in vivo Stable Isotope Labeling in Humans." Metabolites 9, no. 5: 91.
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.