Next Article in Journal
Metabolomics Contributions to the Discovery of Prostate Cancer Biomarkers
Next Article in Special Issue
Urinary Metabolomics Validates Metabolic Differentiation Between Renal Cell Carcinoma Stages and Reveals a Unique Metabolic Profile for Oncocytomas
Previous Article in Journal
Ozone and Wounding Stresses Differently Alter the Temporal Variation in Formylated Phloroglucinols in Eucalyptus globulus Leaves
Previous Article in Special Issue
Crosstalk between Metabolic Alterations and Altered Redox Balance in PTC-Derived Cell Lines

Defining Metabolic Rewiring in Lung Squamous Cell Carcinoma

Aberystwyth University, Institute of Biological, Environmental and Rural Sciences (IBERS), Ceredigion SY23 3DA, UK
São Paulo State University (UNESP), Faculty of Medicine, Dept. of Surgery and Orthopedics, Botucatu 18618687, Brazil
São Paulo State University (UNESP), Experimental Research Unity (UNIPEX), Botucatu 18618687, Brazil
São Paulo State University (UNESP), School of Veterinary Medicine and Animal Science, Dept. of Veterinary Clinic, Botucatu 18618687, Brazil
Clinical Research Centre, Prince Philip Hospital, Hywel Dda University Health Board, Wales SA14 8QF, UK
School of Medicine, Swansea University, Singleton Park, Swansea, Wales SA2 8PP, UK
Authors to whom correspondence should be addressed.
Metabolites 2019, 9(3), 47;
Received: 8 February 2019 / Revised: 26 February 2019 / Accepted: 2 March 2019 / Published: 7 March 2019
(This article belongs to the Special Issue Cancer Metabolomics 2019)
Metabolomics based on untargeted flow infusion electrospray ionization high-resolution mass spectrometry (FIE-HRMS) can provide a snap-shot of metabolism in living cells. Lung Squamous Cell Carcinoma (SCC) is one of the predominant subtypes of Non-Small Cell Lung Cancers (NSCLCs), which usually shows a poor prognosis. We analysed lung SCC samples and matched histologically normal lung tissues from eight patients. Metabolites were profiled by FIE-HRMS and assessed using t-test and principal component analysis (PCA). Differentially accumulating metabolites were mapped to pathways using the mummichog algorithm in R, and biologically meaningful patterns were indicated by Metabolite Set Enrichment Analysis (MSEA). We identified metabolic rewiring networks, including the suppression of the oxidative pentose pathway and found that the normal tricarboxylic acid (TCA) cycle were decoupled from increases in glycolysis and glutamine reductive carboxylation. Well-established associated effects on nucleotide, amino acid and thiol metabolism were also seen. Novel aspects in SCC tissue were increased in Vitamin B complex cofactors, serotonin and a reduction of γ-aminobutyric acid (GABA). Our results show the value of FIE-HRMS as a high throughput screening method that could be exploited in clinical contexts. View Full-Text
Keywords: lung squamous cell carcinoma; flow infusion electrospray ionization high resolution mass spectrometry; untargeted metabolites; pathways lung squamous cell carcinoma; flow infusion electrospray ionization high resolution mass spectrometry; untargeted metabolites; pathways
Show Figures

Figure 1

MDPI and ACS Style

Paes de Araújo, R.; Bertoni, N.; Seneda, A.L.; Felix, T.F.; Carvalho, M.; Lewis, K.E.; Hasimoto, É.N.; Beckmann, M.; Drigo, S.A.; Reis, P.P.; Mur, L.A.J. Defining Metabolic Rewiring in Lung Squamous Cell Carcinoma. Metabolites 2019, 9, 47.

AMA Style

Paes de Araújo R, Bertoni N, Seneda AL, Felix TF, Carvalho M, Lewis KE, Hasimoto ÉN, Beckmann M, Drigo SA, Reis PP, Mur LAJ. Defining Metabolic Rewiring in Lung Squamous Cell Carcinoma. Metabolites. 2019; 9(3):47.

Chicago/Turabian Style

Paes de Araújo, Rachel, Natália Bertoni, Ana L. Seneda, Tainara F. Felix, Márcio Carvalho, Keir E. Lewis, Érica N. Hasimoto, Manfred Beckmann, Sandra A. Drigo, Patricia P. Reis, and Luis A. J. Mur. 2019. "Defining Metabolic Rewiring in Lung Squamous Cell Carcinoma" Metabolites 9, no. 3: 47.

Find Other Styles
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

Back to TopTop