Next Article in Journal
Metabolic Alterations in Male-Sterile Potato as Compared to Male-Fertile
Next Article in Special Issue
Defining Metabolic Rewiring in Lung Squamous Cell Carcinoma
Previous Article in Journal
Metabolic Modeling of Human Gut Microbiota on a Genome Scale: An Overview
Article Menu
Issue 2 (February) cover image

Export Article

Open AccessArticle
Metabolites 2019, 9(2), 23; https://doi.org/10.3390/metabo9020023

Crosstalk between Metabolic Alterations and Altered Redox Balance in PTC-Derived Cell Lines

1
Department of Biomedical Sciences, University of Cagliari, 09124 Cagliari, Italy
2
Department of Biochemistry and Cambridge Systems Biology Centre, University of Cambridge, Cambridge CB2 1GA, UK
*
Author to whom correspondence should be addressed.
Equally contributing.
Received: 20 December 2018 / Revised: 23 January 2019 / Accepted: 29 January 2019 / Published: 1 February 2019
(This article belongs to the Special Issue Cancer Metabolomics 2019)
Full-Text   |   PDF [2971 KB, uploaded 13 February 2019]   |  

Abstract

Background: Thyroid cancer is the most common endocrine malignancy, with papillary thyroid carcinoma (PTC) being the most common (85–90%) among all the different types of thyroid carcinomas. Cancer cells show metabolic alterations and, due to their rapid proliferation, an accumulation of reactive oxygen species, playing a fundamental role in cancer development and progression. Currently, the crosstalk among thyrocytes metabolism, redox balance and oncogenic mutations remain poorly characterized. The aim of this study was to investigate the interplay among metabolic alterations, redox homeostasis and oncogenic mutations in PTC-derived cells. Methods: Metabolic and redox profile, glutamate-cysteine ligase, glutaminase-1 and metabolic transporters were evaluated in PTC-derived cell lines with distinguished genetic background (TPC-1, K1 and B-CPAP), as well as in an immortalized thyroid cell line (Nthy-ori3-1) selected as control. Results: PTC-derived cells, particularly B-CPAP cells, harboring BRAF, TP53 and human telomerase reverse transcriptase (hTERT) mutation, displayed an increase of metabolites and transporters involved in energetic pathways. Furthermore, all PTC-derived cells showed altered redox homeostasis, as reported by the decreased antioxidant ratios, as well as the increased levels of intracellular oxidant species. Conclusion: Our findings confirmed the pivotal role of the metabolism and redox state regulation in the PTC biology. Particularly, the most perturbed metabolic phenotypes were found in B-CPAP cells, which are characterized by the most aggressive genetic background. View Full-Text
Keywords: papillary thyroid carcinoma; metabolomics; metabolic profile; oxidative stress; cancer cell metabolism papillary thyroid carcinoma; metabolomics; metabolic profile; oxidative stress; cancer cell metabolism
Figures

Graphical abstract

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).

Supplementary material

SciFeed

Share & Cite This Article

MDPI and ACS Style

Tronci, L.; Caria, P.; Frau, D.V.; Liggi, S.; Piras, C.; Murgia, F.; Santoru, M.L.; Pibiri, M.; Deiana, M.; Griffin, J.L.; Vanni, R.; Atzori, L. Crosstalk between Metabolic Alterations and Altered Redox Balance in PTC-Derived Cell Lines. Metabolites 2019, 9, 23.

Show more citation formats Show less citations formats

Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]
Metabolites EISSN 2218-1989 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top