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Metabolites 2019, 9(2), 19; https://doi.org/10.3390/metabo9020019

HR-MAS NMR Based Quantitative Metabolomics in Breast Cancer

1
Leibniz Institut für Analytische Wissenschaften, ISAS, e.V., 44139 Dortmund, Germany
2
Leibniz Research Centre for Working Environment and Human Factors (IfADo), 44139 Dortmund, Germany
*
Author to whom correspondence should be addressed.
These authors contributed equally to this work.
Received: 16 December 2018 / Revised: 17 January 2019 / Accepted: 18 January 2019 / Published: 22 January 2019
(This article belongs to the Special Issue Cancer Metabolomics 2018)
Full-Text   |   PDF [2410 KB, uploaded 31 January 2019]   |  

Abstract

High resolution magic-angle spinning (HR-MAS) nuclear magnetic resonance (NMR) spectroscopy is increasingly used for profiling of breast cancer tissue, delivering quantitative information for approximately 40 metabolites. One unique advantage of the method is that it can be used to analyse intact tissue, thereby requiring only minimal sample preparation. Importantly, since the method is non-destructive, it allows further investigations of the same specimen using for instance transcriptomics. Here, we discuss technical aspects critical for a successful analysis—including sample handling, measurement conditions, pulse sequences for one- and two dimensional analysis, and quantification methods—and summarize available studies, with a focus on significant associations of metabolite levels with clinically relevant parameters. View Full-Text
Keywords: NMR; HR MAS; breast cancer; metabolomics NMR; HR MAS; breast cancer; metabolomics
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Gogiashvili, M.; Nowacki, J.; Hergenröder, R.; Hengstler, J.G.; Lambert, J.; Edlund, K. HR-MAS NMR Based Quantitative Metabolomics in Breast Cancer. Metabolites 2019, 9, 19.

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