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Open AccessArticle

Lipidomic Analysis of Plastidial Octanoyltransferase Mutants of Arabidopsis thaliana

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Instituto de la Grasa-CSIC, Building 46, UPO Campus, Ctra. de Utrera km 1, 41013 Seville, Spain
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Alliance Sorbonne Université, Université de Technologie de Compiègne, Génie Enzymatique et Cellulaire (GEC), UMR-CNRS 7025, CS 60319, 60203 Compiègne CEDEX, France
*
Author to whom correspondence should be addressed.
Metabolites 2019, 9(10), 209; https://doi.org/10.3390/metabo9100209
Received: 12 September 2019 / Revised: 27 September 2019 / Accepted: 27 September 2019 / Published: 29 September 2019
(This article belongs to the Special Issue Metabolomic and Flux Analysis in Plants)
Plant de novo fatty acid synthesis takes place in the plastid using acetyl-coenzyme A (acetyl-CoA) as the main precursor. This first intermediate is produced from pyruvate through the action of the plastidial pyruvate dehydrogenase complex (PDH), which catalyses the oxidative decarboxylation of pyruvate to produce acetyl-CoA, CO2, and NADH. For the proper functioning of this complex, lipoic acid is required to be bound to the dihydrolipoamide S-acetyltransferase E2 subunit of PDH. Octanoyltransferase (LIP2; EC 2.3.1.181) and lipoyl synthase (LIP1; EC 2.8.1.8) are the enzymes involved in the biosynthesis of this essential cofactor. In Arabidopsis plastids, an essential lipoyl synthase (AtLIP1p) and two redundant octanoyltransferases (AtLIP2p1 and AtLIP2p2) have been described. In the present study, the lipidomic characterization of Arabidopsis octanoyltransferase mutants reveals new insight into the lipoylation functions within plastid metabolism. Lipids and fatty acids from mature seeds and seedlings from Atlip2p1 and Atlip2p2 mutants were analysed by gas chromatography (GC) and liquid chromatography–electrospray ionization high-resolution mass spectrometry (LC-ESI-HRMS2), the analysis revealed changes in fatty acid profiles that showed similar patterns in both mutant seeds and seedlings and in the lipid species containing those fatty acids. Although both mutants showed similar tendencies, the lack of the AtLIP2p2 isoform produced a more acute variation in its lipids profile. These changes in fatty acid composition and the increase in their content per seed point to the interference of octanoyltransferases in the fatty acid synthesis flux in Arabidopsis thaliana seeds. View Full-Text
Keywords: octanoyltransferases; protein lipoylation; triacylglycerol species; polar lipids; fatty acid biosynthesis octanoyltransferases; protein lipoylation; triacylglycerol species; polar lipids; fatty acid biosynthesis
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Martins-Noguerol, R.; Moreno-Pérez, A.J.; Acket, S.; Makni, S.; Garcés, R.; Troncoso-Ponce, A.; Salas, J.J.; Thomasset, B.; Martínez-Force, E. Lipidomic Analysis of Plastidial Octanoyltransferase Mutants of Arabidopsis thaliana. Metabolites 2019, 9, 209.

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