Tear Film Amphiphilic and Anti-Inflammatory Lipids in Bovine Pink Eye
AbstractBackground: Tear film fluid serves as a dynamic barrier that both lubricates the eye and protects against allergens and infectious agents. However, a detailed analysis of a bacteria-induced immune response on the tear film lipidome has not been undertaken. Methods: We undertook a high-resolution mass spectrometry lipidomics analysis of endogenous anti-inflammatory and structural tear film lipids in bovine pink eye. Results: Bovine pink eye resulted in dramatic elevations in tear fluid levels of the anti-inflammatory lipids resolvin E2, cyclic phosphatidic acid 16:0, and cyclic phosphatidic acid 18:0. In addition, there were elevated levels of the structural lipids (O-acyl)-ω-hydroxy-fatty acids, cholesterol sulfate, ethanolamine plasmalogens, and sphingomyelins. Lipid peroxidation also was augmented in pink eye as evidenced by the hydroperoxy derivatives of ethanolamine plasmalogens. Conclusions: Ocular infections with Moraxella bovis result in the induction of a number of endogenous anti-inflammatory lipids and augmentation of the levels of structural glycerophospholipids and sphingolipids. Increased levels of hydroperoxy glycerophospholipids also indicate that this bacterial infection results in lipid peroxidation. View Full-Text
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Wood, P.L.; Donohue, M.N.; Cebak, J.E.; Beckmann, T.G.; Treece, M.; Johnson, J.W.; Miller, L.M.J. Tear Film Amphiphilic and Anti-Inflammatory Lipids in Bovine Pink Eye. Metabolites 2018, 8, 81.
Wood PL, Donohue MN, Cebak JE, Beckmann TG, Treece M, Johnson JW, Miller LMJ. Tear Film Amphiphilic and Anti-Inflammatory Lipids in Bovine Pink Eye. Metabolites. 2018; 8(4):81.Chicago/Turabian Style
Wood, Paul L.; Donohue, Michelle N.; Cebak, John E.; Beckmann, Taylor G.; Treece, MacKenzie; Johnson, Jason W.; Miller, Lynda M.J. 2018. "Tear Film Amphiphilic and Anti-Inflammatory Lipids in Bovine Pink Eye." Metabolites 8, no. 4: 81.
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