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Metabolites 2018, 8(4), 81; https://doi.org/10.3390/metabo8040081

Tear Film Amphiphilic and Anti-Inflammatory Lipids in Bovine Pink Eye

1
Metabolomics Unit, College of Veterinary Medicine, Lincoln Memorial University, 6965 Cumberland Gap Pkwy, Harrogate TN 37752, UK
2
Department of Medicine, DeBusk College of Osteopathic Medicine, Lincoln Memorial University, 6965 Cumberland Gap Pkwy, Harrogate TN 37752, UK
3
College of Veterinary Medicine, Lincoln Memorial University, 6965 Cumberland Gap Pkwy, Harrogate TN 37752, UK
*
Author to whom correspondence should be addressed.
Received: 15 October 2018 / Revised: 8 November 2018 / Accepted: 16 November 2018 / Published: 21 November 2018
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Abstract

Background: Tear film fluid serves as a dynamic barrier that both lubricates the eye and protects against allergens and infectious agents. However, a detailed analysis of a bacteria-induced immune response on the tear film lipidome has not been undertaken. Methods: We undertook a high-resolution mass spectrometry lipidomics analysis of endogenous anti-inflammatory and structural tear film lipids in bovine pink eye. Results: Bovine pink eye resulted in dramatic elevations in tear fluid levels of the anti-inflammatory lipids resolvin E2, cyclic phosphatidic acid 16:0, and cyclic phosphatidic acid 18:0. In addition, there were elevated levels of the structural lipids (O-acyl)-ω-hydroxy-fatty acids, cholesterol sulfate, ethanolamine plasmalogens, and sphingomyelins. Lipid peroxidation also was augmented in pink eye as evidenced by the hydroperoxy derivatives of ethanolamine plasmalogens. Conclusions: Ocular infections with Moraxella bovis result in the induction of a number of endogenous anti-inflammatory lipids and augmentation of the levels of structural glycerophospholipids and sphingolipids. Increased levels of hydroperoxy glycerophospholipids also indicate that this bacterial infection results in lipid peroxidation. View Full-Text
Keywords: bovine pink eye; infectious bovine keratoconjunctivitis (IBK), resolvin E2; cyclic phosphatidic acid; (O-acyl)-ω-hydroxy-fatty acids; plasmalogens; sphingomyelins; ocular inflammation; tear film fluid bovine pink eye; infectious bovine keratoconjunctivitis (IBK), resolvin E2; cyclic phosphatidic acid; (O-acyl)-ω-hydroxy-fatty acids; plasmalogens; sphingomyelins; ocular inflammation; tear film fluid
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).
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Wood, P.L.; Donohue, M.N.; Cebak, J.E.; Beckmann, T.G.; Treece, M.; Johnson, J.W.; Miller, L.M.J. Tear Film Amphiphilic and Anti-Inflammatory Lipids in Bovine Pink Eye. Metabolites 2018, 8, 81.

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