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Article

Metabolic Perturbations in a Bacillus subtilis clpP Mutant during Glucose Starvation

1
Institute of Biochemistry, University of Greifswald, 17487 Greifswald, Germany
2
Imaging Center of the Department of Biology, University of Greifswald, 17487 Greifswald, Germany
3
Institute of Microbiology, University of Greifswald, 17487 Greifswald, Germany
*
Author to whom correspondence should be addressed.
Metabolites 2017, 7(4), 63; https://doi.org/10.3390/metabo7040063
Received: 7 September 2017 / Revised: 19 November 2017 / Accepted: 21 November 2017 / Published: 24 November 2017
Proteolysis is essential for all living organisms to maintain the protein homeostasis and to adapt to changing environmental conditions. ClpP is the main protease in Bacillus subtilis, and forms complexes with different Clp ATPases. These complexes play crucial roles during heat stress, but also in sporulation or cell morphology. Especially enzymes of cell wall-, amino acid-, and nucleic acid biosynthesis are known substrates of the protease ClpP during glucose starvation. The aim of this study was to analyze the influence of a clpP mutation on the metabolism in different growth phases and to search for putative new ClpP substrates. Therefore, B. subtilis 168 cells and an isogenic ∆clpP mutant were cultivated in a chemical defined medium, and the metabolome was analyzed by a combination of 1H-NMR, HPLC-MS, and GC-MS. Additionally, the cell morphology was investigated by electron microscopy. The clpP mutant showed higher levels of most glycolytic metabolites, the intermediates of the citric acid cycle, amino acids, and peptidoglycan precursors when compared to the wild-type. A strong secretion of overflow metabolites could be detected in the exo-metabolome of the clpP mutant. Furthermore, a massive increase was observed for the teichoic acid metabolite CDP-glycerol in combination with a swelling of the cell wall. Our results show a recognizable correlation between the metabolome and the corresponding proteome data of B. subtilis clpP mutant. Moreover, our results suggest an influence of ClpP on Tag proteins that are responsible for teichoic acids biosynthesis. View Full-Text
Keywords: Clp proteases; cell wall metabolism; metabolism under glucose starvation Clp proteases; cell wall metabolism; metabolism under glucose starvation
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MDPI and ACS Style

Schultz, D.; Schlüter, R.; Gerth, U.; Lalk, M. Metabolic Perturbations in a Bacillus subtilis clpP Mutant during Glucose Starvation. Metabolites 2017, 7, 63. https://doi.org/10.3390/metabo7040063

AMA Style

Schultz D, Schlüter R, Gerth U, Lalk M. Metabolic Perturbations in a Bacillus subtilis clpP Mutant during Glucose Starvation. Metabolites. 2017; 7(4):63. https://doi.org/10.3390/metabo7040063

Chicago/Turabian Style

Schultz, Daniel, Rabea Schlüter, Ulf Gerth, and Michael Lalk. 2017. "Metabolic Perturbations in a Bacillus subtilis clpP Mutant during Glucose Starvation" Metabolites 7, no. 4: 63. https://doi.org/10.3390/metabo7040063

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