Next Article in Journal / Special Issue
Affinity Purification of O-Acetylserine(thiol)lyase from Chlorella sorokiniana by Recombinant Proteins from Arabidopsis thaliana
Previous Article in Journal
Metabolite Profiling of Root Exudates of Common Bean under Phosphorus Deficiency
Previous Article in Special Issue
NblA1/A2-Dependent Homeostasis of Amino Acid Pools during Nitrogen Starvation in Synechocystis sp. PCC 6803
Article Menu

Export Article

Open AccessArticle
Metabolites 2014, 4(3), 612-628;

Carbon Partitioning in Green Algae (Chlorophyta) and the Enolase Enzyme

Department of Biology, Brooklyn College of the City University of New York, 2900 Bedford Avenue 200NE, Brooklyn, NY 11210, USA
The Graduate Center of the City University of New York, 2900 Bedford Avenue 200NE, Brooklyn, NY 11210, USA
Author to whom correspondence should be addressed.
Received: 13 June 2014 / Revised: 25 July 2014 / Accepted: 28 July 2014 / Published: 4 August 2014
(This article belongs to the Special Issue Metabolism in Phototrophic Prokaryotes and Algae)
PDF [399 KB, uploaded 6 August 2014]


The exact mechanisms underlying the distribution of fixed carbon within photoautotrophic cells, also referred to as carbon partitioning, and the subcellular localization of many enzymes involved in carbon metabolism are still unknown. In contrast to the majority of investigated green algae, higher plants have multiple isoforms of the glycolytic enolase enzyme, which are differentially regulated in higher plants. Here we report on the number of gene copies coding for the enolase in several genomes of species spanning the major classes of green algae. Our genomic analysis of several green algae revealed the presence of only one gene coding for a glycolytic enolase [EC]. Our predicted cytosolic localization would require export of organic carbon from the plastid to provide substrate for the enolase and subsequent re-import of organic carbon back into the plastids. Further, our comparative sequence study of the enolase and its 3D-structure prediction may suggest that the N-terminal extension found in green algal enolases could be involved in regulation of the enolase activity. In summary, we propose that the enolase represents one of the crucial regulatory bottlenecks in carbon partitioning in green algae. View Full-Text
Keywords: green algae; metabolism; carbon partitioning; enolase green algae; metabolism; carbon partitioning; enolase

Graphical abstract

This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

Share & Cite This Article

MDPI and ACS Style

Polle, J.E.W.; Neofotis, P.; Huang, A.; Chang, W.; Sury, K.; Wiech, E.M. Carbon Partitioning in Green Algae (Chlorophyta) and the Enolase Enzyme. Metabolites 2014, 4, 612-628.

Show more citation formats Show less citations formats

Related Articles

Article Metrics

Article Access Statistics



[Return to top]
Metabolites EISSN 2218-1989 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top