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Article

Effects of Inorganic Carbon Limitation on the Metabolome of the Synechocystis sp. PCC 6803 Mutant Defective in glnB Encoding the Central Regulator PII of Cyanobacterial C/N Acclimation

1
Universität Rostock, Institut Biowissenschaften, Pflanzenphysiologie, Albert-Einstein-Str. 3, D-18059 Rostock, Germany
2
Max-Planck-Institut für Molekulare Pflanzenphysiologie, Am Mühlenberg 1, 14476 Golm, Germany
*
Author to whom correspondence should be addressed.
These authors contributed equally to this work.
Metabolites 2014, 4(2), 232-247; https://doi.org/10.3390/metabo4020232
Received: 27 February 2014 / Revised: 14 April 2014 / Accepted: 16 April 2014 / Published: 22 April 2014
(This article belongs to the Special Issue Metabolism in Phototrophic Prokaryotes and Algae)
Cyanobacteria are the only prokaryotes performing oxygenic photosynthesis. Non-diazotrophic strains such as the model Synechocystis sp. PCC 6803 depend on a balanced uptake and assimilation of inorganic carbon and nitrogen sources. The internal C/N ratio is sensed via the PII protein (GlnB). We analyzed metabolic changes of the DglnB mutant of Synechocystis sp. PCC 6803 under different CO2 availability. The identified metabolites provided a snapshot of the central C/N metabolism. Cells of the DglnB mutant shifted to carbon-limiting conditions, i.e. a decreased C/N ratio, showed changes in intermediates of the sugar storage and particularly of the tricarboxylic acid cycle, arginine, and glutamate metabolism. The changes of the metabolome support the notion that the PII protein is primarily regulating the N-metabolism whereas the changes in C-metabolism are probably secondary effects of the PII deletion. View Full-Text
Keywords: Inorganic carbon; PII-mutant; Primary metabolism; Metabolic fingerprinting; Synechocystis Inorganic carbon; PII-mutant; Primary metabolism; Metabolic fingerprinting; Synechocystis
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MDPI and ACS Style

Schwarz, D.; Orf, I.; Kopka, J.; Hagemann, M. Effects of Inorganic Carbon Limitation on the Metabolome of the Synechocystis sp. PCC 6803 Mutant Defective in glnB Encoding the Central Regulator PII of Cyanobacterial C/N Acclimation. Metabolites 2014, 4, 232-247. https://doi.org/10.3390/metabo4020232

AMA Style

Schwarz D, Orf I, Kopka J, Hagemann M. Effects of Inorganic Carbon Limitation on the Metabolome of the Synechocystis sp. PCC 6803 Mutant Defective in glnB Encoding the Central Regulator PII of Cyanobacterial C/N Acclimation. Metabolites. 2014; 4(2):232-247. https://doi.org/10.3390/metabo4020232

Chicago/Turabian Style

Schwarz, Doreen, Isabel Orf, Joachim Kopka, and Martin Hagemann. 2014. "Effects of Inorganic Carbon Limitation on the Metabolome of the Synechocystis sp. PCC 6803 Mutant Defective in glnB Encoding the Central Regulator PII of Cyanobacterial C/N Acclimation" Metabolites 4, no. 2: 232-247. https://doi.org/10.3390/metabo4020232

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