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Targeted Chiral Analysis of Bioactive Arachidonic Acid Metabolites Using Liquid-Chromatography-Mass Spectrometry

Centers for Cancer Pharmacology and Excellence in Environmental Toxicology, Department of Pharmacology, University of Pennsylvania School of Medicine, Philadelphia, PA 19104, USA
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Metabolites 2012, 2(2), 337-365; https://doi.org/10.3390/metabo2020337
Received: 1 March 2012 / Revised: 2 April 2012 / Accepted: 9 April 2012 / Published: 20 April 2012
(This article belongs to the Special Issue Analytical Techniques in Metabolomics)
A complex structurally diverse series of eicosanoids arises from the metabolism of arachidonic acid. The metabolic profile is further complicated by the enantioselectivity of eicosanoid formation and the variety of regioisomers that arise. In order to investigate the metabolism of arachidonic acid in vitro or in vivo, targeted methods are advantageous in order to distinguish between the complex isomeric mixtures that can arise by different metabolic pathways. Over the last several years this targeted approach has become more popular, although there are still relatively few examples where chiral targeted approaches have been employed to directly analyze complex enantiomeric mixtures. To efficiently conduct targeted eicosanoid analyses, LC separations are coupled with collision induced dissociation (CID) and tandem mass spectrometry (MS/MS). Product ion profiles are often diagnostic for particular regioisomers. The highest sensitivity that can be achieved involves the use of selected reaction monitoring/mass spectrometry (SRM/MS); whereas the highest specificity is obtained with an SRM transitions between an intense parent ion, which contains the intact molecule (M) and a structurally significant product ion. This review article provides an overview of arachidonic acid metabolism and targeted chiral methods that have been utilized for the analysis of the structurally diverse eicosanoids that arise. View Full-Text
Keywords: arachidonic acid, eicosanoids, chiral liquid chromatography, tandem mass spectrometry, selected reaction monitoring, atmospheric pressure chemical ionization, electron capture, cyclooxygenase, lipoxygenase, cytochrome P-450 arachidonic acid, eicosanoids, chiral liquid chromatography, tandem mass spectrometry, selected reaction monitoring, atmospheric pressure chemical ionization, electron capture, cyclooxygenase, lipoxygenase, cytochrome P-450
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Mesaros, C.; Blair, I.A. Targeted Chiral Analysis of Bioactive Arachidonic Acid Metabolites Using Liquid-Chromatography-Mass Spectrometry. Metabolites 2012, 2, 337-365.

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