Metabolic Profiling Early Post-Allogeneic Haematopoietic Cell Transplantation in the Context of CMV Infection
Abstract
:1. Introduction
2. Materials and Methods
2.1. Patient and Sample Selection
2.2. Sample Selection
2.3. Clinical Data
2.4. Conditioning Regimens
2.5. CMV Monitoring and Treatment
2.6. Metabolomics and Lipidomics Analysis
2.7. Pre-Processing of Metabolomics and Lipidomics Data
2.8. Statistical Analysis
2.9. Pathway-Resolved Correlation Network Analysis
2.10. Software
3. Results
3.1. aHSCT Patient Cohort
3.2. Metabolomics and Lipidomics Data
3.3. Single-Marker Associations with CMV Infection
3.4. WGCNA Module Associations with Clinical Traits
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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All (n = 68) | Cases (n = 34) | Controls (n = 34) | p-Value | |
---|---|---|---|---|
Male * | 46 (72) | 25 (74) | 25 (71) | 1 † |
Age upon aHSCT | 56.5 (17, 73) | 55.5 (22, 70) | 59.5 (17, 73) | 0.84 ‡ |
Sample collection in days post-aHSCT | 28 (8, 33) | 26.8 (14, 33) | 28 (8, 33) | 0.34 ‡ |
CMV infection onset in days post-aHSCT | 48.5 (34, 90) | 48.5 (34, 90) | - | |
Graft origin | 0.19 § | |||
Bone marrow | 11 (16) | 8 (24) | 3 (9) | |
Peripheral blood | 57 (84) | 26 (76) | 31 (91) | |
Conditioning regimen * | 1 † | |||
Myeloablative conditioning (MAC) | 28 (41) | 14 (41) | 14 (41) | |
Non-myeloablative conditioning (Mini) | 40 (59) | 20 (59) | 20 (59) | |
CMV risk score ** | 0.00067 § | |||
Low | 19 (28) | 3 (9) | 16 (47) | |
Intermediate | 21 (31) | 11 (32) | 10 (29) | |
High | 28 (41.2) | 20 (58.8) | 8 (24) |
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Share and Cite
Rasmussen, K.K.; dos Santos, Q.; MacPherson, C.R.; Zucco, A.G.; Gjærde, L.K.; Ilett, E.E.; Lodding, I.; Helleberg, M.; Lundgren, J.D.; Nielsen, S.D.; et al. Metabolic Profiling Early Post-Allogeneic Haematopoietic Cell Transplantation in the Context of CMV Infection. Metabolites 2023, 13, 968. https://doi.org/10.3390/metabo13090968
Rasmussen KK, dos Santos Q, MacPherson CR, Zucco AG, Gjærde LK, Ilett EE, Lodding I, Helleberg M, Lundgren JD, Nielsen SD, et al. Metabolic Profiling Early Post-Allogeneic Haematopoietic Cell Transplantation in the Context of CMV Infection. Metabolites. 2023; 13(9):968. https://doi.org/10.3390/metabo13090968
Chicago/Turabian StyleRasmussen, Kirstine K., Quenia dos Santos, Cameron Ross MacPherson, Adrian G. Zucco, Lars Klingen Gjærde, Emma E. Ilett, Isabelle Lodding, Marie Helleberg, Jens D. Lundgren, Susanne D. Nielsen, and et al. 2023. "Metabolic Profiling Early Post-Allogeneic Haematopoietic Cell Transplantation in the Context of CMV Infection" Metabolites 13, no. 9: 968. https://doi.org/10.3390/metabo13090968