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Metabolites
Volume 13
Issue 3
10.3390/metabo13030425
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Article

Metabolomic Analysis Demonstrates the Impacts of Polyketide Synthases PKS14 and PKS15 on the Production of Beauvericins, Bassianolide, Enniatin A, and Ferricrocin in Entomopathogen Beauveria bassiana

by
Wachiraporn Toopaang
1,2,3,
Kullyanee Panyawicha
3,4,
Chettida Srisuksam
1,
Wei-Chen Hsu
3,5,
Ching-Chih Lin
3,5,
Morakot Tanticharoen
6,
Yu-Liang Yang
3,5,* and
Alongkorn Amnuaykanjanasin
1,*
1
National Center for Genetic Engineering and Biotechnology (BIOTEC), National Science and Technology Development Agency (NSTDA), 113 Thailand Science Park, Paholyothin Rd., Khlong Nueng, Khlong Luang, Pathum Thani 12120, Thailand
2
Molecular and Biological Agricultural Sciences, Taiwan International Graduate Program, Academia Sinica and National Chung Hsing University, Taiwan
3
Agricultural Biotechnology Research Center, Academia Sinica, Taipei 11529, Taiwan
4
Institute of Biotechnology, College of Bioresources and Agriculture, National Taiwan University, Taipei 10617, Taiwan
5
Biotechnology Center in Southern Taiwan, Academia Sinica, Tainan 711010, Taiwan
6
School of Bioresources and Technology, King Mongkut’s University of Technology Thonburi, Bangkok 10140, Thailand
*
Authors to whom correspondence should be addressed.
Metabolites 2023, 13(3), 425; https://doi.org/10.3390/metabo13030425
Submission received: 5 January 2023 / Revised: 4 March 2023 / Accepted: 9 March 2023 / Published: 14 March 2023
(This article belongs to the Special Issue Application of Mass Spectrometry Analysis in Metabolomics)
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Abstract

Beauveria bassiana is a globally distributed entomopathogenic fungus that produces various secondary metabolites to support its pathogenesis in insects. Two polyketide synthase genes, pks14 and pks15, are highly conserved in entomopathogenic fungi and are important for insect virulence. However, understanding of their mechanisms in insect pathogenicity is still limited. Here, we overexpressed these two genes in B. bassiana and compared the metabolite profiles of pks14 and pks15 overexpression strains to those of their respective knockout strains in culture and in vivo using tandem liquid chromatography-mass spectrometry (LC-MS/MS) with Global Natural Products Social Molecular Networking (GNPS). The pks14 and pks15 clusters exhibited crosstalk with biosynthetic clusters encoding insect-virulent metabolites, including beauvericins, bassianolide, enniatin A, and the intracellular siderophore ferricrocin under certain conditions. These secondary metabolites were upregulated in the pks14-overexpressing strain in culture and the pks15-overexpressing strain in vivo. These data suggest that pks14 and pks15, their proteins or their cluster components might be directly or indirectly associated with key pathways in insect pathogenesis of B. bassiana, particularly those related to secondary metabolism. Information about interactions between the polyketide clusters and other biosynthetic clusters improves scientific understanding about crosstalk among biosynthetic pathways and mechanisms of pathogenesis.
Keywords: Beauveria bassiana; polyketide synthase; nonribosomal peptides; GNPS; molecular networking Beauveria bassiana; polyketide synthase; nonribosomal peptides; GNPS; molecular networking
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MDPI and ACS Style

Toopaang, W.; Panyawicha, K.; Srisuksam, C.; Hsu, W.-C.; Lin, C.-C.; Tanticharoen, M.; Yang, Y.-L.; Amnuaykanjanasin, A. Metabolomic Analysis Demonstrates the Impacts of Polyketide Synthases PKS14 and PKS15 on the Production of Beauvericins, Bassianolide, Enniatin A, and Ferricrocin in Entomopathogen Beauveria bassiana. Metabolites 2023, 13, 425. https://doi.org/10.3390/metabo13030425

AMA Style

Toopaang W, Panyawicha K, Srisuksam C, Hsu W-C, Lin C-C, Tanticharoen M, Yang Y-L, Amnuaykanjanasin A. Metabolomic Analysis Demonstrates the Impacts of Polyketide Synthases PKS14 and PKS15 on the Production of Beauvericins, Bassianolide, Enniatin A, and Ferricrocin in Entomopathogen Beauveria bassiana. Metabolites. 2023; 13(3):425. https://doi.org/10.3390/metabo13030425

Chicago/Turabian Style

Toopaang, Wachiraporn, Kullyanee Panyawicha, Chettida Srisuksam, Wei-Chen Hsu, Ching-Chih Lin, Morakot Tanticharoen, Yu-Liang Yang, and Alongkorn Amnuaykanjanasin. 2023. "Metabolomic Analysis Demonstrates the Impacts of Polyketide Synthases PKS14 and PKS15 on the Production of Beauvericins, Bassianolide, Enniatin A, and Ferricrocin in Entomopathogen Beauveria bassiana" Metabolites 13, no. 3: 425. https://doi.org/10.3390/metabo13030425

APA Style

Toopaang, W., Panyawicha, K., Srisuksam, C., Hsu, W.-C., Lin, C.-C., Tanticharoen, M., Yang, Y.-L., & Amnuaykanjanasin, A. (2023). Metabolomic Analysis Demonstrates the Impacts of Polyketide Synthases PKS14 and PKS15 on the Production of Beauvericins, Bassianolide, Enniatin A, and Ferricrocin in Entomopathogen Beauveria bassiana. Metabolites, 13(3), 425. https://doi.org/10.3390/metabo13030425

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