Next Article in Journal
Metabolomic and Transcriptomic Profiling Identified Significant Genes in Thymic Epithelial Tumor
Next Article in Special Issue
The Quantitative Profiling of Oxylipins from Arachidonic Acid by LC-MS/MS in Feces at Birth 3 Days and 21 Days of Piglets
Previous Article in Journal
NMR-Based Metabolomics Identify Metabolic Change in Spleen of Idiopathic Thrombocytopenic Purpura Patients
Previous Article in Special Issue
Sanitary Conditions on the Farm Alters Fecal Metabolite Profile in Growing Pigs
 
 
Article

Comparative Metabolomics of Small Molecules Specifically Expressed in the Dorsal or Ventral Marginal Zones in Vertebrate Gastrula

1
Institute for Advanced Biosciences, Keio University, Tsuruoka 997-0052, Yamagata, Japan
2
Systems Biology Program, Graduate School of Media and Governance, Keio University, Fujisawa 252-0882, Kanagawa, Japan
*
Author to whom correspondence should be addressed.
Academic Editors: Michal Ciborowski, Joanna Godzien and Stanislaw Deja
Metabolites 2022, 12(6), 566; https://doi.org/10.3390/metabo12060566
Received: 30 May 2022 / Revised: 14 June 2022 / Accepted: 17 June 2022 / Published: 20 June 2022
(This article belongs to the Special Issue Animal and Cellular Models in Metabolomics Research Volume 2)
Many previous studies have reported the various proteins specifically secreted as inducers in the dorsal or ventral regions in vertebrate gastrula. However, little is known about the effect on cell fate of small molecules below 1000 Da. We therefore tried to identify small molecules specifically expressed in the dorsal marginal zone (DMZ) or ventral marginal zone (VMZ) in vertebrate gastrula. Small intracellular and secreted molecules were detected using explants and supernatant samples. Hydrophilic metabolites were analyzed by capillary ion chromatography–mass spectrometry and liquid chromatography–mass spectrometry, and lipids were analyzed by supercritical fluid chromatography–tandem mass spectrometry. In total, 190 hydrophilic metabolites and 396 lipids were identified. The DMZ was found to have high amounts of glycolysis- and glutathione metabolism-related metabolites in explants, and the VMZ was richer in purine metabolism-related metabolites. We also discovered some hydrophilic metabolites and lipids differentially contained in the DMZ or VMZ. Our research would contribute to a deeper understanding of the cellular physiology that regulates early embryogenesis. View Full-Text
Keywords: metabolomic analysis; Xenopus laevis; dorsal–ventral patterning; Spemann organizer; early embryogenesis metabolomic analysis; Xenopus laevis; dorsal–ventral patterning; Spemann organizer; early embryogenesis
Show Figures

Graphical abstract

MDPI and ACS Style

Suzuki, Y.; Hayasaka, R.; Hasebe, M.; Ikeda, S.; Soga, T.; Tomita, M.; Hirayama, A.; Kuroda, H. Comparative Metabolomics of Small Molecules Specifically Expressed in the Dorsal or Ventral Marginal Zones in Vertebrate Gastrula. Metabolites 2022, 12, 566. https://doi.org/10.3390/metabo12060566

AMA Style

Suzuki Y, Hayasaka R, Hasebe M, Ikeda S, Soga T, Tomita M, Hirayama A, Kuroda H. Comparative Metabolomics of Small Molecules Specifically Expressed in the Dorsal or Ventral Marginal Zones in Vertebrate Gastrula. Metabolites. 2022; 12(6):566. https://doi.org/10.3390/metabo12060566

Chicago/Turabian Style

Suzuki, Yukako, Ryosuke Hayasaka, Masako Hasebe, Satsuki Ikeda, Tomoyoshi Soga, Masaru Tomita, Akiyoshi Hirayama, and Hiroki Kuroda. 2022. "Comparative Metabolomics of Small Molecules Specifically Expressed in the Dorsal or Ventral Marginal Zones in Vertebrate Gastrula" Metabolites 12, no. 6: 566. https://doi.org/10.3390/metabo12060566

Find Other Styles
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Back to TopTop