1H-NMR-Based Metabonomics Study to Reveal the Progressive Metabolism Regulation of SAP Deficiency on ApoE−/− Mice
Abstract
:1. Introduction
2. Materials and Methods
2.1. Laboratory Animals
2.2. Blood Collection
2.3. 1H-NMR Spectroscopy
2.4. Pattern Recognition and Statistical Analysis
3. Results
3.1. Metabolite Profiling of Serum Samples
3.2. Multivariate Statistical Analysis
3.3. Analysis of Serum Differential Metabolites in ApoE−/− Mice
3.4. Metabolic Regulation of SAP Deficiency in ApoE−/− Mice
3.5. High Fat Diet Has No Effect on the Metabolic Regulation of SAP Deficiency
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Metabolites | δ 1H (ppm) | Pathway | 8 w | 8 + 4 w | 8 + 8 w | ||||||
---|---|---|---|---|---|---|---|---|---|---|---|
ApoE−/− vs. C57 | ApoE−/−;SAP−/− vs. ApoE−/− | ApoE−/−;SAP−/− vs. C57 | ApoE−/− vs. C57 | ApoE−/−;SAP−/− vs. ApoE−/− | ApoE−/−;SAP−/− vs. C57 | ApoE−/− vs. C57 | ApoE−/−;SAP−/− vs. ApoE−/− | ApoE−/−;SAP−/− vs. C57 | |||
Formate | 8.46 | Ketone metabolism | ↓ ** | - | ↓ * | ↓ * | - | ↓ * | ↓ ** | - | ↓ * |
Tyrosine | 6.89, 7.81 | Amino acid metabolism | ↓ *** | ↑ ** | ↓ * | ↓ *** | - | ↓ ** | ↓ ** | - | ↓ * |
Fumarate | 6.51 | Energy metabolism | ↓ *** | - | ↓ ** | ↓ *** | - | ↓ ** | ↓ ** | - | ↓ ** |
Histidine | 7.04, 7.74 | Amino acid metabolism | ↓ *** | - | ↓ ** | ↓ *** | - | ↓ ** | ↓ *** | - | ↓ ** |
Citrate | 2.54 | Energy metabolism | - | - | - | ↓ ** | - | ↓ * | ↓ * | - | ↓ ** |
Acetoacetate | 2.28 | Energy metabolism | ↓ ** | ↑ ** | - | - | - | - | - | - | - |
Acetate | 1.92 | Ketone metabolism | ↓ * | ↑ * | - | ↓ ** | ↑ ** | - | ↓ ** | ↑ * | - |
Pyruvate | 2.37 | Energy metabolism | ↑ ** | ↓ ** | - | ↑ * | ↓ ** | - | ↑ *** | ↓ *** | - |
Choline | 3.2 | Lipid metabolism | ↓ ** | ↑ * | - | ↓ ** | ↑ * | - | ↓ ** | ↑ ** | - |
VLDL + LDL | 0.88, 1.28 | Lipid metabolism | ↑ ** | ↓ ** | - | ↑ ** | ↓ * | - | ↑ ** | ↓ ** | - |
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Li, Q.; Chen, W.; Huang, W.; Hou, R.; Huang, X.; Xu, M.; Que, L.; Wang, L.; Yang, Y. 1H-NMR-Based Metabonomics Study to Reveal the Progressive Metabolism Regulation of SAP Deficiency on ApoE−/− Mice. Metabolites 2022, 12, 1278. https://doi.org/10.3390/metabo12121278
Li Q, Chen W, Huang W, Hou R, Huang X, Xu M, Que L, Wang L, Yang Y. 1H-NMR-Based Metabonomics Study to Reveal the Progressive Metabolism Regulation of SAP Deficiency on ApoE−/− Mice. Metabolites. 2022; 12(12):1278. https://doi.org/10.3390/metabo12121278
Chicago/Turabian StyleLi, Qian, Wanting Chen, Wenbin Huang, Ranran Hou, Xinping Huang, Man Xu, Limei Que, Lijing Wang, and Yongxia Yang. 2022. "1H-NMR-Based Metabonomics Study to Reveal the Progressive Metabolism Regulation of SAP Deficiency on ApoE−/− Mice" Metabolites 12, no. 12: 1278. https://doi.org/10.3390/metabo12121278