Feeding Rainbow Trout with Different Types of Non-Starch Polysaccharides: Impacts on Serum Metabolome and Gut Microbiota
Abstract
:1. Introduction
2. Material and Methods
2.1. Feed Production, Fish and Management
2.2. Sampling Works
2.3. Serum Metabolome Analysis
2.4. Microbiome Analysis
2.5. Statistical Analysis
3. Results
3.1. Quality Control and PCA Analysis
3.2. OPLS-DA Analysis and Differential Metabolite Identification
3.3. Metabolic Pathway Enrichment Analysis of Differential Metabolites
3.4. Microbiota Structure
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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FM | INSP | NSP | NSPs | |
---|---|---|---|---|
Effective Tags | 63,957.00 ± 1300.49 | 64,044.00 ± 3384.14 | 65,748.25 ± 5820.50 | 67,629.75 ± 4018.80 |
Shannon | 6.65 ± 0.25 | 7.36 ± 0.17 | 7.12 ± 1.04 | 6.02 ± 1.29 |
Simpson | 0.94 ± 0.02 | 0.93 ± 0.03 | 0.97 ± 0.01 | 0.88 ± 0.06 |
Chao1 | 1667.05 ± 8.84 | 2762.44 ± 505.06 | 2144.58 ± 126.56 | 2203.73 ± 525.44 |
ACE | 1705.18 ± 51.96 | 2746.35 ± 513.16 | 2179.36 ± 125.66 | 2259.24 ± 531.05 |
Goods coverage | 0.99 ± 0.00 | 0.99 ± 0.00 | 0.99 ± 0.00 | 0.99 ± 0.00 |
Group | R-Value | p-Value |
---|---|---|
FM-INSP | 0.781 | 0.032 |
FM-SNSP | 1.000 | 0.021 |
FM-NSP | 0.583 | 0.028 |
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Zhou, H.; Liu, Y.; Fan, J.; Huang, H.; Deng, J.; Tan, B. Feeding Rainbow Trout with Different Types of Non-Starch Polysaccharides: Impacts on Serum Metabolome and Gut Microbiota. Metabolites 2022, 12, 1167. https://doi.org/10.3390/metabo12121167
Zhou H, Liu Y, Fan J, Huang H, Deng J, Tan B. Feeding Rainbow Trout with Different Types of Non-Starch Polysaccharides: Impacts on Serum Metabolome and Gut Microbiota. Metabolites. 2022; 12(12):1167. https://doi.org/10.3390/metabo12121167
Chicago/Turabian StyleZhou, Hang, Yu Liu, Jiongting Fan, Huajing Huang, Junming Deng, and Beiping Tan. 2022. "Feeding Rainbow Trout with Different Types of Non-Starch Polysaccharides: Impacts on Serum Metabolome and Gut Microbiota" Metabolites 12, no. 12: 1167. https://doi.org/10.3390/metabo12121167