Next Article in Journal
l-Lactate: Food for Thoughts, Memory and Behavior
Next Article in Special Issue
Nontargeted Metabolomics as a Screening Tool for Estimating Bioactive Metabolites in the Extracts of 50 Indigenous Korean Plants
Previous Article in Journal
Mycobacterium avium subsp. paratuberculosis Proteome Changes Profoundly in Milk
Previous Article in Special Issue
Metabolite Profiling Reveals Distinct Modulation of Complex Metabolic Networks in Non-Pigmented, Black, and Red Rice (Oryza sativa L.) Cultivars
Article

Characterization of Phytoestrogens in Medicago sativa L. and Grazing Beef Cattle

1
School of Agricultural, Environmental and Veterinary Sciences, Charles Sturt University, Wagga Wagga, NSW 2678, Australia
2
Graham Centre for Agricultural Innovation, Locked Bag 588, Wagga Wagga, NSW 2678, Australia
3
Faculty of Science, National Life Sciences Hub, Building 289, Charles Sturt University, Wagga Wagga, NSW 2678, Australia
4
Department of Agriculture, Falkland Islands Government, Stanley FIQQ 1ZZ, Falkland Islands
*
Author to whom correspondence should be addressed.
Academic Editor: Sandor Gonda
Metabolites 2021, 11(8), 550; https://doi.org/10.3390/metabo11080550
Received: 21 June 2021 / Revised: 13 August 2021 / Accepted: 16 August 2021 / Published: 20 August 2021
(This article belongs to the Special Issue Plant Metabolomics II)
Phytoestrogens are plant-produced bioactive secondary metabolites known to play an integral role in plant defense that frequently accumulate in times of stress and/or microbial infection. Phytoestrogens typically belong to two distinct chemical classes; flavonoids (isoflavones) and non-flavonoids (lignans and coumestans). Upon consumption by livestock, high concentrations of phytoestrogens can cause long-term disruption in reproduction due to structural similarities with mammalian estrogens and their tendency to bind estrogen receptors. Wide variation in phytoestrogen concentration has been reported in pasture legumes and corresponding silage or hay. Lucerne is a common perennial pasture legume in temperate climates, but information on phytoestrogen production or accumulation in grazing livestock is currently limited. Therefore, metabolic profiling using UHPLC-MS-QToF was performed to identify and quantitate key phytoestrogens in both fresh and dried lucerne fodder from replicated field or controlled glasshouse environments. Phytoestrogens were also profiled in the blood plasma of Angus cattle grazing field-grown lucerne. Results revealed that phytoestrogens varied quantitatively and qualitatively among selected lucerne cultivars grown under glasshouse conditions. Fresh lucerne samples contained higher concentrations of coumestans and other phytoestrogenic isoflavones than did dried samples for all cultivars profiled, with several exceeding desirable threshold levels for grazing cattle. Coumestans and isoflavones profiled in plasma of Angus heifers grazing lucerne increased significantly over a 21-day sampling period following experimental initiation. Currently, threshold concentrations for phytoestrogens in plasma are unreported. However, total phytoestrogen concentration exceeded 300 mg·kg−1 in fresh and 180 mg·kg−1 in dried samples of selected cultivars, suggesting that certain genotypes may upregulate phytoestrogen production, while others may prove suitable sources of fodder for grazing livestock. View Full-Text
Keywords: cattle; coumestans; lucerne; mass spectrometry; metabolic profiling; phytoestrogens; reproduction cattle; coumestans; lucerne; mass spectrometry; metabolic profiling; phytoestrogens; reproduction
Show Figures

Figure 1

MDPI and ACS Style

Wyse, J.M.; Latif, S.; Gurusinghe, S.; Berntsen, E.D.; Weston, L.A.; Stephen, C.P. Characterization of Phytoestrogens in Medicago sativa L. and Grazing Beef Cattle. Metabolites 2021, 11, 550. https://doi.org/10.3390/metabo11080550

AMA Style

Wyse JM, Latif S, Gurusinghe S, Berntsen ED, Weston LA, Stephen CP. Characterization of Phytoestrogens in Medicago sativa L. and Grazing Beef Cattle. Metabolites. 2021; 11(8):550. https://doi.org/10.3390/metabo11080550

Chicago/Turabian Style

Wyse, Jessica M., Sajid Latif, Saliya Gurusinghe, Erica D. Berntsen, Leslie A. Weston, and Cyril P. Stephen 2021. "Characterization of Phytoestrogens in Medicago sativa L. and Grazing Beef Cattle" Metabolites 11, no. 8: 550. https://doi.org/10.3390/metabo11080550

Find Other Styles
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Back to TopTop