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Article

TCF19 Impacts a Network of Inflammatory and DNA Damage Response Genes in the Pancreatic β-Cell

1
Division of Endocrinology, Diabetes, and Metabolism, Department of Medicine, University of Wisconsin-Madison, Madison, WI 53705, USA
2
Department of Biological Sciences, Marquette University, Milwaukee, WI 53233, USA
3
Department of Neuroscience, University of Wisconsin-Madison, Madison, WI 53705, USA
4
William S. Middleton Memorial Veterans Hospital, Madison, WI 53705, USA
*
Author to whom correspondence should be addressed.
Academic Editor: Amedeo Lonardo
Metabolites 2021, 11(8), 513; https://doi.org/10.3390/metabo11080513
Received: 18 June 2021 / Revised: 31 July 2021 / Accepted: 2 August 2021 / Published: 4 August 2021
(This article belongs to the Special Issue The Role of β Cells in Diabetes)
Transcription factor 19 (TCF19) is a gene associated with type 1 diabetes (T1DM) and type 2 diabetes (T2DM) in genome-wide association studies. Prior studies have demonstrated that Tcf19 knockdown impairs β-cell proliferation and increases apoptosis. However, little is known about its role in diabetes pathogenesis or the effects of TCF19 gain-of-function. The aim of this study was to examine the impact of TCF19 overexpression in INS-1 β-cells and human islets on proliferation and gene expression. With TCF19 overexpression, there was an increase in nucleotide incorporation without any change in cell cycle gene expression, alluding to an alternate process of nucleotide incorporation. Analysis of RNA-seq of TCF19 overexpressing cells revealed increased expression of several DNA damage response (DDR) genes, as well as a tightly linked set of genes involved in viral responses, immune system processes, and inflammation. This connectivity between DNA damage and inflammatory gene expression has not been well studied in the β-cell and suggests a novel role for TCF19 in regulating these pathways. Future studies determining how TCF19 may modulate these pathways can provide potential targets for improving β-cell survival. View Full-Text
Keywords: DNA damage; inflammation; STRING; RNA-seq; PANTHER; diabetes; β-cell; TCF19 DNA damage; inflammation; STRING; RNA-seq; PANTHER; diabetes; β-cell; TCF19
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MDPI and ACS Style

Yang, G.H.; Fontaine, D.A.; Lodh, S.; Blumer, J.T.; Roopra, A.; Davis, D.B. TCF19 Impacts a Network of Inflammatory and DNA Damage Response Genes in the Pancreatic β-Cell. Metabolites 2021, 11, 513. https://doi.org/10.3390/metabo11080513

AMA Style

Yang GH, Fontaine DA, Lodh S, Blumer JT, Roopra A, Davis DB. TCF19 Impacts a Network of Inflammatory and DNA Damage Response Genes in the Pancreatic β-Cell. Metabolites. 2021; 11(8):513. https://doi.org/10.3390/metabo11080513

Chicago/Turabian Style

Yang, Grace H., Danielle A. Fontaine, Sukanya Lodh, Joseph T. Blumer, Avtar Roopra, and Dawn B. Davis. 2021. "TCF19 Impacts a Network of Inflammatory and DNA Damage Response Genes in the Pancreatic β-Cell" Metabolites 11, no. 8: 513. https://doi.org/10.3390/metabo11080513

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