Capillary Electrophoresis Mass Spectrometry-Based Metabolomics of Plasma Samples from Healthy Subjects in a Cross-Sectional Japanese Population Study
Abstract
:1. Introduction
2. Results and Discussion
2.1. Exploration of Factors Causing Variation in Large-Scale Measurements
2.2. Evaluation of Normalization Using the QC Samples with Smoothing
2.3. Correlation Analysis between Plasma Metabolites and Diagnostic Blood Tests
3. Materials and Methods
3.1. Human Participants and Sample Collection
3.2. Exclusion Criteria for Medical Parameters and Diagnostic Blood Tests
3.3. Metabolome Analysis
3.4. QC Samples and Normalization with Whittaker Smoothing
3.5. Statistical Analysis
4. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Male | Female | Total | |
---|---|---|---|
Sample size | 66 | 122 | 188 |
Age (year) | |||
Mean | 42.9 | 45.1 | 44.3 |
Median | 39.5 | 43.5 | 43 |
Range | 20–74 | 20–73 | 20–74 |
BMI (kg/m2) | |||
Mean | 23.1 | 21.2 | 21.9 |
Median | 22.85 | 21.1 | 21.5 |
Range | 18.2–29 | 15.5–28.1 | 15.5–29 |
Blood Tests | Lower Limit | Upper Limit |
---|---|---|
ALB (g/L) | <41 | >51 |
TG (mmol/L) | <0.47 (M), <0.34 (F) | >2.51 (M), >1.4 (F) |
UA (mmol/L) | <224 (M), <154 (F) | >474 (M), >334 (F) |
GLU (mmol/L) | <4.2 | >5.9 |
γ-GT (U/L) | <9 | >55 |
CRE (mmol/L) | <0.64 (M), <0.46 (F) | >1.06 (M), >0.78 (F), |
C-reactive protein (mg/L) | - | >1.4 |
Hb (g/L) | <135 (M), <110 (F) | >169 (M), >148 (F) |
MCV (fl) | <82 | >98 |
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Yamamoto, H.; Suzuki, M.; Matsuta, R.; Sasaki, K.; Kang, M.-I.; Kami, K.; Tatara, Y.; Itoh, K.; Nakaji, S. Capillary Electrophoresis Mass Spectrometry-Based Metabolomics of Plasma Samples from Healthy Subjects in a Cross-Sectional Japanese Population Study. Metabolites 2021, 11, 314. https://doi.org/10.3390/metabo11050314
Yamamoto H, Suzuki M, Matsuta R, Sasaki K, Kang M-I, Kami K, Tatara Y, Itoh K, Nakaji S. Capillary Electrophoresis Mass Spectrometry-Based Metabolomics of Plasma Samples from Healthy Subjects in a Cross-Sectional Japanese Population Study. Metabolites. 2021; 11(5):314. https://doi.org/10.3390/metabo11050314
Chicago/Turabian StyleYamamoto, Hiroyuki, Makoto Suzuki, Rira Matsuta, Kazunori Sasaki, Moon-Il Kang, Kenjiro Kami, Yota Tatara, Ken Itoh, and Shigeyuki Nakaji. 2021. "Capillary Electrophoresis Mass Spectrometry-Based Metabolomics of Plasma Samples from Healthy Subjects in a Cross-Sectional Japanese Population Study" Metabolites 11, no. 5: 314. https://doi.org/10.3390/metabo11050314