Peer-Review Record
Integrated Metabolomics and Proteomics Dynamics of Serum Samples Reveals Dietary Zeolite Clinoptilolite Supplementation Restores Energy Balance in High Yielding Dairy Cows
Metabolites 2021, 11(12), 842; https://doi.org/10.3390/metabo11120842
by
Sudipa Maity, Ivana Rubić, Josipa Kuleš, Anita Horvatić
, Dražen Đuričić, Marko Samardžija, Blanka Beer Ljubić, Romana Turk, Damjan Gračner, Nino Maćešić, Hrvoje Valpotić and Vladimir Mrljak *
, Dražen Đuričić, Marko Samardžija, Blanka Beer Ljubić, Romana Turk, Damjan Gračner, Nino Maćešić, Hrvoje Valpotić and Vladimir Mrljak *Reviewer 1:
Anonymous
Reviewer 2:
Anonymous
Metabolites 2021, 11(12), 842; https://doi.org/10.3390/metabo11120842
Submission received: 15 November 2021
/
Revised: 2 December 2021
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Accepted: 2 December 2021
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Published: 5 December 2021
(This article belongs to the Section Nutrition and Metabolism)
Round 1
Reviewer 1 Report
- This is a novel study using metabolomics and proteomics to study the effect of zeolite clinoptilolite on the negative energy balance of dairy cows.
- Where is Table S1? Also the BHB and NEFA concentration results if possible BW changes to verify NEB status should be presented in the context.
- Have feed intake and BW change recorded? It is not very convincing to conclude the restoration of energy balance only based on BHB and NEFA concentrations.
Author Response
Please see the attachment
Author Response File: 
Author Response.pdf
Reviewer 2 Report
The manuscript explored the effects of supplementation of Zeolite clinoptilolite on serum metabolome and proteome profiles in peripartal dairy cows. This research article would expand our awareness on the effectiveness of adding mycotoxin binders at the systems biology level in dairy cattle. The manuscript is well-written and the methods are well-described. Please, find my comments below
INTRRODUCTION”
- Change “can result to altered ..” into “can result in altered ..”
- Change “disorder and may take ..” into “disorder and might take ..”
- Cite a reference “..also result in infertility.”
- Change “..have focused in investigating..” into “..have focused on investigating..”
- Define BHB in “..adipose tissue and BHB.”
- Replace “,beta-hydroxybutyrate, “ with “,BHB,”
- Cite a reference in “..mammary epithelial cells during NEB.”
- Cite a reference in “..other biochemical applications.”
- Cite a reference in “..or in diverse pathogeneses.”
- Cite a reference in “..nutrients during the digestion process.”
- Cite a reference in “..energy status of the animals.”
MATERIALS AND METHODS
- Add protocol # after “..University of Zagreb, Croatia.”
- How many heifers and cows in each group?
Author Response
Please see the attachment
Author Response File: Author Response.pdf
