Bariatric Surgery Improves the Atherogenic Profile of Circulating Methylarginines in Obese Patients: Results from a Pilot Study
Abstract
:1. Introduction
2. Results
2.1. Obesity Increased the Serum Concentrations of Methylarginines
2.2. Bariatric Surgery Improved the Atherogenic Profile of Methylarginines
2.3. Correlations of Methylarginine Concentrations with Clinical Data
3. Discussion
4. Materials and Methods
4.1. Subjects
4.2. Chemical and Reagents
4.3. Chemical Synthesis of Dimethylguanidino Valerate
4.4. Biochemical Measurements
4.5. Quantification of Methylarginine Metabolites
4.6. Statistical Analyses
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data availability statement
Acknowledgments
Conflicts of Interest
References
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Healthy Controls | Obese Patients at Baseline | p-Value 1 | |
---|---|---|---|
n | 31 | 31 | - |
Male/Female | 9/22 | 9/22 | 0.713 |
Age, years | 40 ± 10 | 41 ± 9 | 0.652 |
BMI, kg/m2 | 22 ± 3 | 45 ± 6 | 0.003 |
TC, mg/dL | 187 ± 31 | 189 ± 31 | 0.888 |
TG, mg/dL | 81 [45–176] | 108 [59–625] | 0.003 |
HDL-C, mg/dL | 73 [50–90] | 0.003 | |
LDL-C, mg/dL | 95 ± 34 | 120 ± 21 | 0.003 |
Glucose, mg/dL | 77 [58–103] | 93 [72–341] | 0.007 |
Insulin, mIU/L | 15 [3–48] | 19 [6–55] | 0.031 |
HOMA-IR | 2.9 [0.7–12.1] | 4.9 [2.9–23.9] | 0.006 |
ASAT, IU/L | 14 [3–44] | 19 [11–64] | 0.027 |
ALAT, IU/L | 22 [13–98] | 28 [5–114] | 0.005 |
eGFR, mL/min/1.73 m2 | Not available | 99 ± 19 | - |
NAFLD FS | Not available | −0.662 ± 1.259 | - |
6 Months | 12 Months | |||
---|---|---|---|---|
Parameter | % of Change | p-Value 1 | % of Change | p-Value 1 |
BMI | −23.8 | 0.002 | −28.7 | 0.002 |
TC | −9.6 | 0.002 | −11.4 | 0.002 |
TG | −26.4 | 0.004 | −36.2 | 0.004 |
HDL-C | +10.6 | 0.999 | +31.1 | 0.027 |
LDL-C | −11.7 | 0.038 | −18.9 | 0.008 |
Glucose | −17.9 | 0.036 | −21.8 | 0.011 |
Insulin | −55.4 | 0.002 | −63.7 | 0.003 |
HOMA-IR | −62.0 | 0.002 | −71.4 | 0.003 |
ASAT | −16.4 | 0.043 | −15.3 | 0.031 |
ALAT | −30.8 | 0.009 | −30.9 | 0.004 |
eGFR | +11.1 | 0.002 | +5.9 | 0.004 |
NAFLD FS | −66.7 | 0.011 | −92.4 | 0.002 |
Parameter | Healthy Controls | Obese Patients (12 Months Post-Surgery) | p-Value 1 |
---|---|---|---|
n | 31 | 31 | - |
Male/Female | 9/22 | 9/22 | 0.785 |
Age, years | 40 ± 10 | 41 ± 9 | 0.698 |
BMI, kg/m2 | 22 ± 3 | 32 ± 5 | 0.003 |
TC, mg/dL | 187 ± 31 | 167 ± 30 | 0.020 |
TG, mg/dL | 81 [45–176] | 68 [36–125] | 0.020 |
HDL-C, mg/dL | 73 [50–121] | 54 [37–79] | 0.003 |
LDL-C, mg/dL | 95 ± 34 | 97 ± 24 | 0.815 |
Glucose, mg/dL | 77 [58–103] | 83 [70–106] | 0.089 |
Insulin, mIU/L | 15 [3–48] | 8 [2–14] | 0.003 |
HOMA-IR | 2.9 [0.7–12.1] | 1.7 [0.4–2.9] | 0.038 |
ASAT, IU/L | 14 [3–44] | 16 [11–37] | 0.831 |
ALAT, IU/L | 22 [13–98] | 0.020 | |
eGFR, mL/min/1.73 m2 | Not available | - | |
ARG, µmol/L | 157 ± 24 | 16 [5–36] | 0.972 |
NMMA, nmol/L | 466 ± 118 | 105 ± 16 | 0.003 |
CIT, µmol/L | 33 ± 5 | 37 ± 9 | 0.089 |
ADMA, nmol/L | 820 ± 176 | 1066 ± 202 | 0.003 |
SDMA, nmol/L | 685 ± 103 | 800 ± 156 | 0.003 |
DMGV, nmol/L | 324 [87–891] | 483 [245–1251] | 0.003 |
Compound | MRM Transition (m/z) | Cone/Collision (V) |
---|---|---|
ARG | 231.1 → 69.9 | 30/20 |
13C-ARG (IS) | 232.1 → 69.9 | 30/20 |
CIT | 232.1 → 112.9 | 30/20 |
2H7-CIT (IS) | 239.1 → 119.9 | 30/20 |
NMMA | 245.1 → 69.9 | 30/20 |
ADMA | 259.1 → 214.1 | 30/20 |
SDMA | 259.1 → 228.1 | 30/20 |
DMGV | 258.2 → 113.9 | 30/25 |
2H7-ADMA (IS) | 266.2 → 221.1 | 30/20 |
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Poirier, J.; Cloteau, C.; Aguesse, A.; Billot, X.; Thévenot, E.; Krempf, M.; Valéro, R.; Maraninchi, M.; Croyal, M. Bariatric Surgery Improves the Atherogenic Profile of Circulating Methylarginines in Obese Patients: Results from a Pilot Study. Metabolites 2021, 11, 759. https://doi.org/10.3390/metabo11110759
Poirier J, Cloteau C, Aguesse A, Billot X, Thévenot E, Krempf M, Valéro R, Maraninchi M, Croyal M. Bariatric Surgery Improves the Atherogenic Profile of Circulating Methylarginines in Obese Patients: Results from a Pilot Study. Metabolites. 2021; 11(11):759. https://doi.org/10.3390/metabo11110759
Chicago/Turabian StylePoirier, Julie, Chloé Cloteau, Audrey Aguesse, Xavier Billot, Etienne Thévenot, Michel Krempf, René Valéro, Marie Maraninchi, and Mikaël Croyal. 2021. "Bariatric Surgery Improves the Atherogenic Profile of Circulating Methylarginines in Obese Patients: Results from a Pilot Study" Metabolites 11, no. 11: 759. https://doi.org/10.3390/metabo11110759