Strain-Specific Liver Metabolite Profiles in Medaka
Abstract
:1. Introduction
2. Results
2.1. NMR Spectroscopy of Liver Extracts
2.2. Strain-Specific Metabolic Profile of Liver Extracts
2.3. Metabolic Differences between Male and Female Fish Are Not as Pronounced as between the Inbred Strains
3. Discussion
4. Materials and Methods
4.1. Animal Handling and Tissue Collection
4.2. Sample Preparation
4.3. 1H-NMR Spectroscopy of Liver Samples
4.4. Data Analysis
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Both Sexes | Male | Female | ||||
---|---|---|---|---|---|---|
Multivariate | Univariate | Multivariate | Univariate | Multivariate | Univariate | |
VIP Score | FC (p-Value) | VIP Score | FC (p-Value) | VIP Score | FC (p-Value) | |
Aspartate | 1.86 | 1.54 (1.39 × 10−9) | 1.79 | 1.70 (1.62 × 10−7) | 1.67 | 1.41 (0.0012) |
Lactate/Threonine | 1.82 | 0.56 (2.88 × 10−9) | 1.57 | 0.46 (1.51 × 10−5) | 1.87 | 0.67 (1.16 × 10−4) |
ATP | 1.80 | 0.56 (2.90 × 10−9) | 1.71 | 0.48 (9.37 × 10−7) | 1.65 | 0.67 (0.0012) |
u8.51 | 1.66 | 0.54 (1.08 × 10−7) | 1.48 | 0.53 (5.01 × 10−5) | 1.91 | 0.53 (1.16 × 10−4) |
Fumarate | 1.58 | 2.21 (6.94 × 10−7) | 1.52 | 3.09 (2.77 × 10−5) | 1.29 | 1.69 (0.0170) |
Phenylalanine | 1.35 | 1.65 (5.40 × 10−5) | 1.30 | 2.00 (6.78 × 10−4) | 1.09 | 1.39 (0.0428) |
Malate | 1.29 | 1.34 (1.42 × 10−4) | 0.96 | 1.32 (0.0154) | 1.44 | 1.37 (0.0066) |
Ornithine | 1.22 | 1.34 (2.78 × 10−4) | 1.06 | 1.35 (0.0073) | 1.46 | 1.34 (0.0066) |
Tyrosine | 1.22 | 1.49 (2.78 × 10−4) | 1.23 | 1.61 (0.0013) | 1.35 | 1.43 (0.0118) |
Hypotaurine | 1.20 | 5.10 (3.54 × 10−4) | 1.57 | 5.69 (1.51 × 10−5) | 1.10 | 5.09 (0.0428) |
Alanine | 1.19 | 0.78 (3.70 × 10−4) | 1.52 | 0.72 (2.77 × 10−5) | 0.90 | 0.83 (0.0971) |
u2.39 | 1.15 | 1.40 (5.39 × 10−4) | 1.30 | 1.88 (6.78 × 10−4) | 0.56 | 1.10 (0.3076) |
AMP | 1.14 | 1.36 (5.40 × 10−4) | 0.85 | 1.39 (0.0318) | 1.43 | 1.33 (0.0066) |
Choline | 1.06 | 0.79 (0.0013) | 0.72 | 0.87 (0.0741) | 1.28 | 0.72 (0.0170) |
Sarcosine | 1.02 | 0.77 (0.0022) | 0.45 | 0.88 (0.3004) | 1.42 | 0.69 (0.0066) |
Both Strains | HO5 | iCab | ||||
---|---|---|---|---|---|---|
Multivariate | Univariate | Multivariate | Univariate | Multivariate | Univariate | |
VIP Score | FC (p-Value) | VIP Score | FC (p-Value) | VIP Score | FC (p-Value) | |
3-Methylhistidine | 2.31 | 12.77 (2.63 × 10−12) | 2.10 | 12.48 (1.31 × 10−6) | 1.84 | 13.00 (1.46 × 10−5) |
Creatine | 2.28 | 0.42 (3.98 × 10−12) | 1.84 | 0.42 (9.39 × 10−5) | 2.06 | 0.42 (1.64 × 10−7) |
Glutathione_red | 1.68 | 0.65 (1.11 × 10−5) | 1.33 | 0.76 (0.0114) | 1.55 | 0.55 (0.0006) |
Glutamine | 1.57 | 0.80 (5.15 × 10−5) | 1.83 | 0.75 (9.39 × 10−5) | 0.94 | 0.85 (0.0579) |
Tyrosine | 1.47 | 0.66 (0.0002) | 1.49 | 0.69 (0.0037) | 1.32 | 0.61 (0.0053) |
Glycerophosphocholine | 1.45 | 1.39 (0.0002) | 0.82 | 1.19 (0.1397) | 1.57 | 1.60 (0.0005) |
Ornithine | 1.36 | 0.75 (0.0006) | 1.23 | 0.75 (0.0145) | 1.61 | 0.75 (0.0003) |
Hypotaurine | 1.31 | 0.20 (0.0010) | 1.05 | 0.20 (0.0433) | 1.72 | 0.18 (8.43 × 10−5) |
Formate | 1.29 | 0.16 (0.0010) | 1.07 | 0.20 (0.0394) | 1.27 | 0.16 (0.0061) |
Nicotinamide | 1.28 | 1.57 (0.0010) | 1.27 | 1.56 (0.0125) | 1.04 | 1.59 (0.0343) |
Alanine | 1.19 | 0.80 (0.0022) | 1.51 | 0.74 (0.0033) | 0.79 | 0.86 (0.1132) |
u8.51 | 1.16 | 1.46 (0.0028) | 1.36 | 1.47 (0.0091) | 1.27 | 1.47 (0.0061) |
Aspartate | 0.75 | 0.86 (0.0743) | 0.52 | 0.92 (0.4048) | 1.30 | 0.76 (0.0058) |
ATP | 0.74 | 1.23 (0.0749) | 0.03 | 1.01 (0.9656) | 1.33 | 1.39 (0.0053) |
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Soergel, H.; Loosli, F.; Muhle-Goll, C. Strain-Specific Liver Metabolite Profiles in Medaka. Metabolites 2021, 11, 744. https://doi.org/10.3390/metabo11110744
Soergel H, Loosli F, Muhle-Goll C. Strain-Specific Liver Metabolite Profiles in Medaka. Metabolites. 2021; 11(11):744. https://doi.org/10.3390/metabo11110744
Chicago/Turabian StyleSoergel, Hannah, Felix Loosli, and Claudia Muhle-Goll. 2021. "Strain-Specific Liver Metabolite Profiles in Medaka" Metabolites 11, no. 11: 744. https://doi.org/10.3390/metabo11110744