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Article

Serum Metabolomic and Lipoprotein Profiling of Pancreatic Ductal Adenocarcinoma Patients of African Ancestry

1
Department of Surgery, School of Clinical Medicine, Faculty of Health Sciences, University of the Witwatersrand, Johannesburg 2193, South Africa
2
Hepatopancreatobiliary Unit, Department of Surgery, Chris Hani-Baragwanath Academic Hospital, Johannesburg 1864, South Africa
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Centre for Human Metabolomics, Faculty of Natural and Agricultural Sciences, North-West University, Potchefstroom 2531, South Africa
4
Cancer Genomics Group, International Centre for Genetic Engineering and Biotechnology, Cape Town 7925, South Africa
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Institute for Reproductive and Developmental Biology, Imperial College, London SW7 2AZ, UK
*
Author to whom correspondence should be addressed.
Metabolites 2021, 11(10), 663; https://doi.org/10.3390/metabo11100663
Received: 9 August 2021 / Revised: 6 September 2021 / Accepted: 8 September 2021 / Published: 28 September 2021
(This article belongs to the Section Frontiers in Metabolomics)
Pancreatic ductal adenocarcinoma (PDAC) is a lethal cancer with a characteristic dysregulated metabolism. Abnormal clinicopathological features linked to defective metabolic and inflammatory response pathways can induce PDAC development and progression. In this study, we investigated the metabolites and lipoproteins profiles of PDAC patients of African ancestry. Nuclear Magnetic Resonance (NMR) spectroscopy was conducted on serum obtained from consenting individuals (34 PDAC, 6 Chronic Pancreatitis, and 6 healthy participants). Seventy-five signals were quantified from each NMR spectrum. The Liposcale test was used for lipoprotein characterization. Spearman’s correlation and Kapan Meier tests were conducted for correlation and survival analyses, respectively. In our patient cohort, the results demonstrated that levels of metabolites involved in the glycolytic pathway increased with the tumour stage. Raised ethanol and 3-hydroxybutyrate were independently correlated with a shorter patient survival time, irrespective of tumour stage. Furthermore, increased levels of bilirubin resulted in an abnormal lipoprotein profile in PDAC patients. Additionally, we observed that the levels of a panel of metabolites (such as glucose and lactate) and lipoproteins correlated with those of inflammatory markers. Taken together, the metabolic phenotype can help distinguish PDAC severity and be used to predict patient survival and inform treatment intervention. View Full-Text
Keywords: pancreatic ductal adenocarcinoma; metabolites; cholestatic (obstructive) jaundice; lipoprotein; inflammation; tumour stages pancreatic ductal adenocarcinoma; metabolites; cholestatic (obstructive) jaundice; lipoprotein; inflammation; tumour stages
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MDPI and ACS Style

Elebo, N.; Omoshoro-Jones, J.; Fru, P.N.; Devar, J.; De Wet van Zyl, C.; Vorster, B.C.; Smith, M.; Cacciatore, S.; Zerbini, L.F.; Candy, G.; Nweke, E.E. Serum Metabolomic and Lipoprotein Profiling of Pancreatic Ductal Adenocarcinoma Patients of African Ancestry. Metabolites 2021, 11, 663. https://doi.org/10.3390/metabo11100663

AMA Style

Elebo N, Omoshoro-Jones J, Fru PN, Devar J, De Wet van Zyl C, Vorster BC, Smith M, Cacciatore S, Zerbini LF, Candy G, Nweke EE. Serum Metabolomic and Lipoprotein Profiling of Pancreatic Ductal Adenocarcinoma Patients of African Ancestry. Metabolites. 2021; 11(10):663. https://doi.org/10.3390/metabo11100663

Chicago/Turabian Style

Elebo, Nnenna, Jones Omoshoro-Jones, Pascaline N. Fru, John Devar, Christiaan De Wet van Zyl, Barend C. Vorster, Martin Smith, Stefano Cacciatore, Luiz F. Zerbini, Geoffrey Candy, and Ekene E. Nweke 2021. "Serum Metabolomic and Lipoprotein Profiling of Pancreatic Ductal Adenocarcinoma Patients of African Ancestry" Metabolites 11, no. 10: 663. https://doi.org/10.3390/metabo11100663

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