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Metabolite and Phytohormone Profiling Illustrates Metabolic Reprogramming as an Escape Strategy of Deepwater Rice during Partially Submerged Stress

1
RIKEN Center for Sustainable Resource Science, Yokohama, Kanagawa 230-0045, Japan
2
Bioscience and Biotechnology Center, Nagoya University, Nagoya, Aichi 464-8601, Japan
3
Faculty of Agriculture, Yamagata University, Tsuruoka, Yamagata 997-8555, Japan
4
Graduate School of Bioagricultural Sciences, Nagoya University, Nagoya, Aichi 464-8601, Japan
5
Graduate School of Pharmaceutical Sciences, Chiba University, Chuo-ku, Chiba 263-8522, Japan
6
Graduate School of Life and Environmental Sciences, University of Tsukuba, Tsukuba, Ibaraki 305-8572, Japan
7
Tsukuba Plant Innovation Research Center, University of Tsukuba, Tsukuba, Ibaraki 305-8572, Japan
*
Author to whom correspondence should be addressed.
Present address: Division of Applied Genetics, Institute of Agrobiological Sciences, National Agriculture and Food Research Organization (NARO), Tsukuba, Ibaraki 305-8634, Japan.
Metabolites 2020, 10(2), 68; https://doi.org/10.3390/metabo10020068 (registering DOI)
Received: 29 December 2019 / Revised: 3 February 2020 / Accepted: 11 February 2020 / Published: 14 February 2020
(This article belongs to the Special Issue Plant Metabolomics)
Rice varieties that can survive under submergence conditions respond to flooding either by enhancing internode elongation or by quiescence of shoot elongation. Despite extensive efforts to identify key metabolites triggered by complete submergence of rice possessing SUBMERGENCE 1 (SUB1) locus, metabolic responses of internode elongation of deepwater rice governed by the SNORKEL 1 and 2 genes remain elusive. This study investigated specific metabolomic responses under partial submergence (PS) to deepwater- (C9285) and non-deepwater rice cultivars (Taichung 65 (T65)). In addition, we examined the response in a near-isogenic line (NIL-12) that has a C9285 genomic fragment on chromosome 12 introgressed into the genetic background of T65. Under short-term submergence (0–24 h), metabolite profiles of C9285, NIL-12, and T65 were compared to extract significantly changed metabolites in deepwater rice under PS conditions. Comprehensive metabolite and phytohormone profiling revealed increases in metabolite levels in the glycolysis pathway in NIL-12 plants. Under long-term submergence (0–288 h), we found decreased amino acid levels. These metabolomic changes were opposite when compared to those in flood-tolerant rice with SUB1 locus. Auxin conjugate levels related to stress response decreased in NIL-12 lines relative to T65. Our analysis helped clarify the complex metabolic reprogramming in deepwater rice as an escape strategy. View Full-Text
Keywords: deepwater rice; anoxia; hypoxia; SNORKEL; flooding stress; metabolome; glycolysis deepwater rice; anoxia; hypoxia; SNORKEL; flooding stress; metabolome; glycolysis
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Fukushima, A.; Kuroha, T.; Nagai, K.; Hattori, Y.; Kobayashi, M.; Nishizawa, T.; Kojima, M.; Utsumi, Y.; Oikawa, A.; Seki, M.; Sakakibara, H.; Saito, K.; Ashikari, M.; Kusano, M. Metabolite and Phytohormone Profiling Illustrates Metabolic Reprogramming as an Escape Strategy of Deepwater Rice during Partially Submerged Stress. Metabolites 2020, 10, 68.

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