Chemical Labeling Assisted Detection and Identification of Short Chain Fatty Acid Esters of Hydroxy Fatty Acid in Rat Colon and Cecum Contents
Abstract
:1. Introduction
2. Results and Discussion
2.1. Chemical Labeling Assisted Detection and Identification of SFAHFAs
2.2. Annotation of DMED-labeled-SFAHFAs by MS/MS Analysis
2.3. Fragmentation Pattern of DMED-labeled SFAHFAs
2.4. Altered Levels of SFAHFAs as Possible Biomarkers of Obesity
3. Materials and Methods
3.1. Chemicals
3.2. Animal Samples
3.3. Sample Preparation for LC-MS
3.4. LC-MS Analysis
3.5. Statistical Analysis
4. Conclusions
Supplementary Materials
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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SI No | Type of SFAHFA | Theoretical m/z | Experimental m/z | Mass Error ppm | Identified |
---|---|---|---|---|---|
1 | SFAHFA 2:0/20:0 | 441.4056 | 441.4042 | −3.1 | [12] |
2 | SFAHFA 3:0/20:0 | 455.4213 | 455.4198 | −3.2 | This study |
3 | SFAHFA 24:1 | 467.4213 | 467.4197 | −3.4 | [12] |
4 | SFAHFA 2:0/22:0 or 3:0/21:0 | 469.4369 | 469.4355 | −2.9 | [12] |
5 | SFAHFA 3:0/22:1 | 481.4369 | 481.4355 | −2.9 | This study |
6 | SFAHFA 3:0/22:0 | 483.4526 | 483.4524 | −0.4 | [12] |
7 | SFAHFA 2:0/24:2 | 493.4369 | 493.4352 | −3.4 | This study |
8 | SFAHFA 2:0/24:1 or 3:0/23:1 | 495.4526 | 495.4507 | −3.8 | [12] |
9 | SFAHFA 2:0/24:0 or 3:0/23:0 | 497.4682 | 497.4677 | −1.0 | [12] |
10 | SFAHFA 3:0/24:2 | 507.4526 | 507.4514 | −2.3 | [12] |
11 | SFAHFA 3:0/24:1 | 509.4682 | 509.4673 | −1.7 | [12] |
12 | SFAHFA 3:0/24:0 or 2:0/25:0 | 511.4839 | 511.4833 | −1.1 | [12] |
13 | SFAHFA 28:1 | 523.4839 | 523.4830 | −1.7 | This study |
14 | SFAHFA 28:0 | 525.4995 | 525.4989 | −1.1 | [12] |
15 | SFAHFA 29:1 | 537.4995 | 537.4990 | −0.9 | This study |
16 | SFAHFA 29:0 | 539.5152 | 539.5150 | −0.4 | This study |
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B. Gowda, S.G.; Gowda, D.; Liang, C.; Li, Y.; Kawakami, K.; Fukiya, S.; Yokota, A.; Chiba, H.; Hui, S.-P. Chemical Labeling Assisted Detection and Identification of Short Chain Fatty Acid Esters of Hydroxy Fatty Acid in Rat Colon and Cecum Contents. Metabolites 2020, 10, 398. https://doi.org/10.3390/metabo10100398
B. Gowda SG, Gowda D, Liang C, Li Y, Kawakami K, Fukiya S, Yokota A, Chiba H, Hui S-P. Chemical Labeling Assisted Detection and Identification of Short Chain Fatty Acid Esters of Hydroxy Fatty Acid in Rat Colon and Cecum Contents. Metabolites. 2020; 10(10):398. https://doi.org/10.3390/metabo10100398
Chicago/Turabian StyleB. Gowda, Siddabasave Gowda, Divyavani Gowda, Chongsheng Liang, Yonghan Li, Kentaro Kawakami, Satoru Fukiya, Atsushi Yokota, Hitoshi Chiba, and Shu-Ping Hui. 2020. "Chemical Labeling Assisted Detection and Identification of Short Chain Fatty Acid Esters of Hydroxy Fatty Acid in Rat Colon and Cecum Contents" Metabolites 10, no. 10: 398. https://doi.org/10.3390/metabo10100398