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Study of the Royal Jelly Free Fatty Acids by Liquid Chromatography-High Resolution Mass Spectrometry (LC-HRMS)

Department of Chemistry, National and Kapodistrian University of Athens, 15771 Athens, Greece
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Metabolites 2020, 10(1), 40; https://doi.org/10.3390/metabo10010040
Received: 9 December 2019 / Revised: 11 January 2020 / Accepted: 14 January 2020 / Published: 16 January 2020
(This article belongs to the Special Issue Compound Identification of Small Molecules)
The lipidome of royal jelly (RJ) consists of medium-chained (8–12 carbon atoms) free fatty acids. We present herein a liquid chromatography-high resolution mass spectrometry (HRMS) method that permits the determination of RJ fatty acids and at the same time the detection of suspect fatty acids. The method allows for the direct quantification of seven free fatty acids of RJ, avoiding any derivatization step. It was validated and applied in seven RJ samples, where the major RJ fatty acid trans-10-hydroxy-2-decenoic acid (10-HDA) was found to vary from 0.771 ± 0.08 to 0.928 ± 0.04 g/100 g fresh RJ. Four additional suspect fatty acids were simultaneously detected taking advantage of the HRMS detection.
Keywords: 10-Hydroxy-2-decenoic acid; fatty acids; high resolution mass spectrometry; royal jelly 10-Hydroxy-2-decenoic acid; fatty acids; high resolution mass spectrometry; royal jelly
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Kokotou, M.G.; Mantzourani, C.; Babaiti, R.; Kokotos, G. Study of the Royal Jelly Free Fatty Acids by Liquid Chromatography-High Resolution Mass Spectrometry (LC-HRMS). Metabolites 2020, 10, 40.

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