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Scientia Pharmaceutica is published by MDPI from Volume 84 Issue 3 (2016). Articles in this Issue were published by another publisher in Open Access under a CC-BY (or CC-BY-NC-ND) licence. Articles are hosted by MDPI on as a courtesy and upon agreement with Austrian Pharmaceutical Society (Österreichische Pharmazeutische Gesellschaft, ÖPhG).
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Sci. Pharm. 2009, 77(2), 367-374;

Simultaneous Determination of Epinephrine and Norepinephrine by High Performance Liquid Chromatography

Cellular and Microbiology Laboratory, College of Pharmacy, IFTM, Lodhipur Rajput - 243112, Moradabad - 244001, Uttar Pradesh, India
Author to whom correspondence should be addressed.
Received: 15 February 2009 / Accepted: 2 April 2009 / Published: 4 April 2009
PDF [215 KB, uploaded 21 December 2016]


Epinephrine and non-epinephrine are major endogenous catecholamines which are known as neurotransmitter. The plasma levels of catecholamines are significant markers of several neuro-endocrine disorders and autonomic nervous system disorders. A method for the simultaneous quantitation of epinephrine and norepinephrine is described. The related substances are resolved on a Phenomenex ODS analytical column C18 column (150 mm × 4.6 mm, 5 μm) using a mobile phase composed of a mixture of acetic acid and 50mM sodium acetate buffer pH 3.1 (1:99 v/v) at a flow rate of 1 ml/min with UV detector at 285 nm. The method is compatible with HPLC-MS and provides a tool for the control of substandard and counterfeit commercial preparations of epinephrine and norepinephrine.
Keywords: Adrenaline; Noraderaniline; HPLC Adrenaline; Noraderaniline; HPLC
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).

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MISHRA, A.; UPADHYAY, A.; PATRA, A.; CHAUDHURY, S.; CHATTOPADHYAY, P. Simultaneous Determination of Epinephrine and Norepinephrine by High Performance Liquid Chromatography. Sci. Pharm. 2009, 77, 367-374.

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