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Scientia Pharmaceutica is published by MDPI from Volume 84 Issue 3 (2016). Articles in this Issue were published by another publisher in Open Access under a CC-BY (or CC-BY-NC-ND) licence. Articles are hosted by MDPI on mdpi.com as a courtesy and upon agreement with Austrian Pharmaceutical Society (Österreichische Pharmazeutische Gesellschaft, ÖPhG).
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A Simple Kinetic Spectrophotometric Method For The Determination of Certain 4-Quinolones in Drug Formulations.

Department of Analytical, Chemistry, Faculty of Pharmacy, University of Mansoura, Mansoura, 35516, Egypt
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Sci. Pharm. 2000, 68(2), 173-188; https://doi.org/10.3797/scipharm.aut-00-16
Received: 14 March 2000 / Revised: 9 May 2000 / Accepted: 9 May 2000 / Published: 10 May 2000
An accurate simple and selective kinetic procedure for the determination of certain 4-quinolones namely, norfloxacin (I), ofloxacin (II), enrofloxacin (III), fleroxacin (IV), ciprofloxacin (V) and pefloxacin (VI) is described. The procedure is based on reacting the studied compounds in acidic media (0.1 M HCL) with 3-methyl-2-benzothiazolinone hydrazone hydrochloride (MBTH) in presence of cerium (IV) ammonium sulphate as an oxidant at room temperature for a fixed time of 20 min. for (I), (III), 12 min for (II) and 30 min for IV , V and VI, then the absorbance of the reaction product is measured at 630 nm. The concentration of the studied compounds is computed using the corresponding calibration curve equation for the fixed-time method. The absorbance-concentrations plot is rectilinear over the range 20-100 ug.ml-1 for (I), 2 - 20 ug.ml-1 for (II), 10 - 74 ug.ml-1 for (III), 10 - 60 ug.ml-1 for IV, 10 - 50 ug.ml-1 for IV and 8 - 40 ug.ml-1 for VI. The determination of the studied compounds by the fixed-concentration and rate constant methods is feasible with the calibration equation obtained, but the fixed-time method proved to be more applicable. The procedure was applied successfully to commercial tablets and ampoules and the results obtained were compared statistically with the reference methods.
Keywords: Kinetic determination; 4-quinolone antibiotics; 3-methyl-2-benzothiazolinone hydrazone HCL (MBTH); Ce. (IV); pharmaceutical analysis Kinetic determination; 4-quinolone antibiotics; 3-methyl-2-benzothiazolinone hydrazone HCL (MBTH); Ce. (IV); pharmaceutical analysis
MDPI and ACS Style

Rizk, M.; Belal, F.; Ibrahim, F.; Ahmed, S.M.; El-Enany, N.M. A Simple Kinetic Spectrophotometric Method For The Determination of Certain 4-Quinolones in Drug Formulations.. Sci. Pharm. 2000, 68, 173-188.

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