Cosmetic Potential of a Recombinant 50 kDa Protein
Abstract
:1. Introduction
2. Materials and Methods
2.1. Formulation Compatibility Evaluation
2.2. EpiOcular
2.3. Bacterial Reverse Mutation Assay
2.4. MTT Assay
2.5. Type I Collagen Assay
2.6. Type III Collagen Assays
3. Results
3.1. Characterization of 50 kDa Protein
3.2. Formulation Testing and Potential Applications
3.3. Safety Testing
3.4. Collagen Synthesis Induction in Human Primary Dermal Fibroblast by 50 kDa Protein
4. Discussion
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Major Category | Specific Examples |
---|---|
Skincare | Eye care—Cream, serum, mask (sheet, pad, cream, etc..), treatment Eyebrow—serums, primer and treatment Eyelash—serums, primer and treatment |
Face care
| |
Lip care (tinted or not, with/without SPF)—lip oil, lip balm, lip mask, lip treatment | |
Body care
| |
Haircare—prewash hair treatment, hair mist, scalp treatment and/or oil, serum, primer, leave-in conditioner, hair mask, hair toner, leave on spray, lotion, haircare shampoo and dry shampoo, wash off conditioner, hair dye, scalp scrub and all haircare styling product | |
Shaving—Aftershave balm, razor burn and bumps relief, bear oil
| |
Makeup |
|
Supplements/drinks |
Ingredients: | %wt/wt |
---|---|
Alcohol-Based Toner | |
Water (Aqua) | Remainder Ingredient |
Recombinant collagen fragment disclosed herein | about 0.0005% to about 25% |
Alcohol Denat. | 10–20% |
Pentylene Glycol | 5–10% |
Glycerin | 1–5% |
Gluconolactone | 0.1–1% |
Dipotassium Glycyrrhizate | 0.1–1% |
Sodium Citrate | 0.1–1% |
Sodium Benzoate | 0.1–1% |
Water-Based Toner | |
Water (Aqua) | Remainder Ingredient |
Recombinant collagen fragment disclosed herein | about 0.0005% to about 25% |
Niacinamide | 1–5% |
Pentylene Glycol | 1–5% |
Propanediol | 1–5% |
Glycerin | 1–5% |
Biosaccharide Gum-1 | 0.1–1% |
Glyceryl Caprylate | 0.1–1% |
Sodium Anisate | 0.1–1% |
Sodium Hydroxide | 0.1–1% |
Caprylhydroxamic Acid | 0.1–1% |
Acrylates/C10-30 Alkyl Acrylate Crosspolymer | 0.1–1% |
Sodium Levulinate | 0.1–1% |
Caprylyl Glycol | 0.1–1% |
Cream | |
Water (Aqua) | Remainder Ingredient |
Recombinant collagen fragment disclosed herein | about 0.0005% to about 25% |
Cetearyl Alcohol | 5–10% |
Glycerin | 1–5% |
Squalane | 1–5% |
Butyrospermum Parkii (Shea) Butter | 1–5% |
Glyceryl Caprylate | 1–5% |
Microcrystalline Cellulose | 1–5% |
Glyceryl Stearate Citrate | 0.1–1% |
Tocopheryl Acetate | 0.1–1% |
Cetearyl Glucoside | 0.1–1% |
Sodium Stearoyl Glutamate | 0.1–1% |
Cellulose Gum | 0.1–1% |
Xanthan Gum | 0.1–1% |
Caprylhydroxamic Acid | 0.1–1% |
Sodium Phytate | 0.01–0.1% |
Gel | |
Water (Aqua) | Remainder Ingredient |
Recombinant collagen fragment disclosed herein | about 0.0005% to about 25% |
Sodium Phytate | 0.1–1% |
Sodium Hydroxide | 0.1–1% |
Carbomer | 0.1–1% |
Phenoxyethanol | 0.1–1% |
Serum | |
Water (Aqua) | Remainder Ingredient |
Recombinant collagen fragment disclosed herein | about 0.0005% to about 25% |
Pentylene Glycol | 1–5% |
Niacinamide | 1–5% |
Dimethicone | 1–5% |
Propanediol | 1–5% |
Tocopherol | 0.1–1% |
Sodium Hyaluronate | 0.1–1% |
Linoleic Acid | 0.1–1% |
Ammonium Acryloyldimethyltaurate/VP Copolymer | 0.1–1% |
Acrylates/C10-30 Alkyl Acrylate Cross polymer | 0.1–1% |
Caprylyl Glyceryl Ether | 0.1–1% |
Tetrasodium Glutamate Diacetate | 0.01–0.1% |
Sodium Hydroxide | 0.01–0.1% |
Phenoxyethanol | 0.01–0.1% |
Linolenic Acid | 0.001–0.0 |
Shampoo | |
Water (Aqua) | Remainder Ingredient |
Recombinant collagen fragment disclosed herein | about 0.0005% to about 25% |
Cocamidopropyl Betaine | 5–10% |
Sodium Lauroyl Methyl Isethionate | 5–10% |
Propanediol | 1–5% |
Sodium Methyl Oleoyl Taurate | 1–5% |
Sodium Cocoyl Isethionate | 1–5% |
Trisodium Ethylenediamine Disuccinate | 0.1–1% |
Caprylhydroxamic Acid | 0.1–1% |
Panthenol | 0.1–1% |
Citric Acid | 0.1–1% |
Caprylyl Glycol | 0.1–1% |
Sodium Benzoate | 0.1–1% |
Conditioner | |
Water (Aqua) | Remainder Ingredient |
Recombinant collagen fragment disclosed herein | about 0.0005% to about 25% |
Cetearyl Alcohol | 5–10% |
Glyceryl Caprylate | 1–5% |
Behentrimonium Methosulfate | 1–5% |
Glycerin | 1–5% |
Caprylhydroxamic Acid | 0.1–1% |
Panthenol | 0.1–1% |
Hydroxyethylcellulose | 0.1–1% |
Cocos Nucifera (Coconut) Oil | 0.1–1% |
Citric Acid | 0.01–0.1% |
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Aly, N.; Benoit, E.; Chaubard, J.-L.; Chintalapudi, K.; Choung, S.; de Leeuw, M.; Diaz, M.; Dueppen, D.; Ferraro, B.; Fischetti, V.; et al. Cosmetic Potential of a Recombinant 50 kDa Protein. Cosmetics 2022, 9, 8. https://doi.org/10.3390/cosmetics9010008
Aly N, Benoit E, Chaubard J-L, Chintalapudi K, Choung S, de Leeuw M, Diaz M, Dueppen D, Ferraro B, Fischetti V, et al. Cosmetic Potential of a Recombinant 50 kDa Protein. Cosmetics. 2022; 9(1):8. https://doi.org/10.3390/cosmetics9010008
Chicago/Turabian StyleAly, Nesma, Emilie Benoit, Jean-Luc Chaubard, Kavyasree Chintalapudi, Soojin Choung, Monique de Leeuw, Matthew Diaz, Dan Dueppen, Bryce Ferraro, Valerie Fischetti, and et al. 2022. "Cosmetic Potential of a Recombinant 50 kDa Protein" Cosmetics 9, no. 1: 8. https://doi.org/10.3390/cosmetics9010008