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Anti-Inflammatory Activity of the Active Compounds of Sanguisorbae Radix In Macrophages and in Vivo Toxicity Evaluation in Zebrafish

1
Gennolab Co., Ltd., Gyeongbuk 38540, Korea
2
Apharm Co., Ltd. 559, Dalseo-daero, Dalseo-gu, Daegu 42709, Korea
3
Department of Cosmeceutical Science, Daegu Haany University, Gyeongbuk 38540, Korea
*
Author to whom correspondence should be addressed.
Cosmetics 2019, 6(4), 68; https://doi.org/10.3390/cosmetics6040068
Received: 29 October 2019 / Revised: 27 November 2019 / Accepted: 29 November 2019 / Published: 4 December 2019
(This article belongs to the Special Issue Anti-oxidant and Anti-inflammatory Properties of Natural Compounds)
Sanguisorbae Radix (SR) is the root of the Sanguisorba officinalis L., a plant native to Asian countries and used in traditional medicine. We isolated the active components of SR and investigated their anti-inflammatory potential. Quercetin (QC), (+)-catechin (CC), and gallic acid (GA) were isolated from acetone extracts of SR. To elucidate the molecular mechanism by which these compounds suppress inflammation, we analyzed the transcriptional up-regulation of inflammatory mediators, such as nuclear factor-kappa B (NF-κB) and its target genes, inducible NOS (iNOS), and cyclooxygenase (COX)-2, in lipopolysaccharide (LPS)-stimulated macrophage RAW264.7 cells. Notably, QC, CC, and GA were found to inhibit the production of nitric oxide, tumor necrosis factor-alpha, and prostaglandin in a dose-dependent manner. Western blot results indicate that the compounds decreased the expression of iNOS and COX-2 proteins. Furthermore, the compounds decreased phosphorylation of IKK, IκB, ERK, p-38, and JNK proteins in LPS-induced cells. The results support the notion that QC, CC, and GA can potently inhibit the inflammatory response, with QC showing the highest anti-inflammatory activity. In in vivo toxicity studies in zebrafish (Danio rerio), QC showed no toxicity up to 25 μg/mL. Therefore, QC has non-toxic potential as a skin anti-inflammatory biomaterial. View Full-Text
Keywords: anti-inflammatory biomaterial; macrophages; Sanguisorba officinalis; Sanguisorbae Radix anti-inflammatory biomaterial; macrophages; Sanguisorba officinalis; Sanguisorbae Radix
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Jang, Y.-A.; Hur, Y.; Lee, J.-T. Anti-Inflammatory Activity of the Active Compounds of Sanguisorbae Radix In Macrophages and in Vivo Toxicity Evaluation in Zebrafish. Cosmetics 2019, 6, 68.

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