Pleurochrysis carterae Hot-Water Extract Inhibits Melanogenesis in Murine Melanoma Cells
1
Division for Animal Science, College of Agriculture, Tamagawa University, 6-1-1 Tamagawa-gakuen, Machida, Tokyo 194-8610, Japan
2
Graduate School of Agriculture, Tamagawa University, 6-1-1 Tamagawa-gakuen, Machida, Tokyo 194-8610, Japan
3
MAC Gifu Research Institute, MicroAlgae Corporation 4-15 Akebono, Gifu 500-8148, Japan
*
Author to whom correspondence should be addressed.
Cosmetics 2019, 6(4), 60; https://doi.org/10.3390/cosmetics6040060
Received: 7 September 2019 / Revised: 15 October 2019 / Accepted: 16 October 2019 / Published: 18 October 2019
In this study, we examined the effect of a hot-water extract of coccolithophore Pleurochrysis carterae on melanogenesis in B16F1 and B16F10 melanoma cells. P. carterae extract inhibited the α-melanocyte-stimulating hormone (α-MSH)-enhanced melanin synthesis in B16F1 melanoma cells. P. carterae also inhibited unstimulated melanin synthesis in B16F10 melanoma cells. Western blotting showed that the P. carterae extract inhibited tyrosinase and microphthalmia-associated transcription factor (MITF) in a dose-dependent manner. The reporter assay also revealed a decline in the tyrosinase promoter activity in the presence of P. carterae extract. Furthermore, quantitative real-time RT-PCR analysis showed that P. carterae extract downregulated the mRNA levels of tyrosinase and MITF. Finally, our study demonstrated that the hot-water extract of P. carterae inhibits melanin synthesis via the down-regulation of MITF mRNA level. Our findings indicate that P. carterae extract could be a possible cosmetic ingredient.
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Keywords:
coccolithophore; Pleurochrysis carterae; microalgae; melanogenesis; tyrosinase; MITF
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MDPI and ACS Style
Sato, K.; Yamaguchi, Y.; Sakaki, S.; Takenaka, H. Pleurochrysis carterae Hot-Water Extract Inhibits Melanogenesis in Murine Melanoma Cells. Cosmetics 2019, 6, 60.
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