Metabolites Produced by Kaistia sp. 32K Promote Biofilm Formation in Coculture with Methylobacterium sp. ME121
Abstract
:1. Introduction
2. Materials and Methods
2.1. Bacterial Strains and Growth Media
2.2. Monoculture and Coculture Conditions
2.3. Preparation of the Culture Supernatant of Strain ME121
2.4. Preparation of Culture Supernatant of Strains 32K and K. adipata
2.5. Biofilm Formation Assay
2.6. Swimming Speed of Strain ME121 and M. radiotolerans in the Culture Supernatant of Strain 32K and K. Adipata
2.7. Statistical Analysis
3. Results
3.1. Biofilm Formation during the Coculture of Bacterial Strains ME121 and 32K
3.2. Biofilm Formation Using Culture Supernatants of Strains ME121 and 32K
3.3. Relationship between Growth and Biofilm Formation during Coculture of Strains ME121 and 32K
3.4. Comparison of the Amount of Biofilm Formed due to Difference in Initial Inoculum Size
3.5. Comparison of Biofilm Formation during Coculture with Another Methylobacterium and Kaistia Strain
3.6. Comparison of Swimming Speed during Coculture with Another Methylobacterium and Kaistia Bacteria
4. Discussion
Relationship between Motility Acceleration of Strain ME121 and Biofilm Formation
Supplementary Materials
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
Ethical Approval
References
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Usui, Y.; Shimizu, T.; Nakamura, A.; Ito, M. Metabolites Produced by Kaistia sp. 32K Promote Biofilm Formation in Coculture with Methylobacterium sp. ME121. Biology 2020, 9, 287. https://doi.org/10.3390/biology9090287
Usui Y, Shimizu T, Nakamura A, Ito M. Metabolites Produced by Kaistia sp. 32K Promote Biofilm Formation in Coculture with Methylobacterium sp. ME121. Biology. 2020; 9(9):287. https://doi.org/10.3390/biology9090287
Chicago/Turabian StyleUsui, Yoshiaki, Tetsu Shimizu, Akira Nakamura, and Masahiro Ito. 2020. "Metabolites Produced by Kaistia sp. 32K Promote Biofilm Formation in Coculture with Methylobacterium sp. ME121" Biology 9, no. 9: 287. https://doi.org/10.3390/biology9090287