Upregulated Wnt-11 and miR-21 Expression Trigger Epithelial Mesenchymal Transition in Aggressive Prostate Cancer Cells

Arisan, Elif Damla; Rencuzogullari, Ozge; Freitas, Ines Lua; Radzali, Syanas; Keskin, Buse; Kothari, Archana; Warford, Antony; Uysal-Onganer, Pinar

doi:10.3390/biology9030052

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Upregulated Wnt-11 and miR-21 Expression Trigger Epithelial Mesenchymal Transition in Aggressive Prostate Cancer Cells

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Institute of Biotechnology, Gebze Technical University, Gebze 41400, Kocaeli, Turkey

Department of Molecular Biology and Genetics, Istanbul Kultur University, Atakoy Campus 34156, Istanbul, Turkey

Cancer Research Group, School of Life Sciences, College of Liberal Arts and Sciences, University of Westminster, 115 New Cavendish Street, London W1W 6UW, UK

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Department of Histopathology, Kingston Hospital, Galsworthy Road, London KT2 7QE, UK

Author to whom correspondence should be addressed.

Biology 2020, 9(3), 52; https://doi.org/10.3390/biology9030052

Received: 15 January 2020 / Revised: 24 February 2020 / Accepted: 6 March 2020 / Published: 9 March 2020

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Abstract

Prostate cancer (PCa) is the second-leading cause of cancer-related death among men. microRNAs have been identified as having potential roles in tumorigenesis. An oncomir, miR-21, is commonly highly upregulated in many cancers, including PCa, and showed correlation with the Wnt-signaling axis to increase invasion. Wnt-11 is a developmentally regulated gene and has been found to be upregulated in PCa, but its mechanism is unknown. The present study aimed to investigate the roles of miR-21 and Wnt-11 in PCa in vivo and in vitro. First, different Gleason score PCa tissue samples were used; both miR-21 and Wnt-11 expressions correlate with high Gleason scores in PCa patient tissues. This data then was confirmed with formalin-fixed paraffin cell blocks using PCa cell lines LNCaP and PC3. Cell survival and colony formation studies proved that miR-21 involves in cells’ behaviors, as well as the epithelial-mesenchymal transition. Consistent with the previous data, silencing miR-21 led to significant inhibition of cellular invasiveness. Overall, these results suggest that miR-21 plays a significant role related to Wnt-11 in the pathophysiology of PCa. View Full-Text

Keywords: microRNA; in situ hybridization; prostate cancer; Wnt-11; miR-21

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MDPI and ACS Style

Arisan, E.D.; Rencuzogullari, O.; Freitas, I.L.; Radzali, S.; Keskin, B.; Kothari, A.; Warford, A.; Uysal-Onganer, P. Upregulated Wnt-11 and miR-21 Expression Trigger Epithelial Mesenchymal Transition in Aggressive Prostate Cancer Cells. Biology 2020, 9, 52. https://doi.org/10.3390/biology9030052

AMA Style

Arisan ED, Rencuzogullari O, Freitas IL, Radzali S, Keskin B, Kothari A, Warford A, Uysal-Onganer P. Upregulated Wnt-11 and miR-21 Expression Trigger Epithelial Mesenchymal Transition in Aggressive Prostate Cancer Cells. Biology. 2020; 9(3):52. https://doi.org/10.3390/biology9030052

Chicago/Turabian Style

Arisan, Elif D., Ozge Rencuzogullari, Ines L. Freitas, Syanas Radzali, Buse Keskin, Archana Kothari, Antony Warford, and Pinar Uysal-Onganer. 2020. "Upregulated Wnt-11 and miR-21 Expression Trigger Epithelial Mesenchymal Transition in Aggressive Prostate Cancer Cells" Biology 9, no. 3: 52. https://doi.org/10.3390/biology9030052

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Upregulated Wnt-11 and miR-21 Expression Trigger Epithelial Mesenchymal Transition in Aggressive Prostate Cancer Cells

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