Upregulated Wnt-11 and miR-21 Expression Trigger Epithelial Mesenchymal Transition in Aggressive Prostate Cancer Cells
by
Elif Damla Arisan 1
, Ozge Rencuzogullari 2, Ines Lua Freitas 3, Syanas Radzali 3, Buse Keskin 2, Archana Kothari 4, Antony Warford 3 and 
Pinar Uysal-Onganer 3,*
Elif Damla Arisan 1, Ozge Rencuzogullari 2, Ines Lua Freitas 3, Syanas Radzali 3, Buse Keskin 2, Archana Kothari 4, Antony Warford 3 and Pinar Uysal-Onganer 3,*
1
Institute of Biotechnology, Gebze Technical University, Gebze 41400, Kocaeli, Turkey
2
Department of Molecular Biology and Genetics, Istanbul Kultur University, Atakoy Campus 34156, Istanbul, Turkey
3
Cancer Research Group, School of Life Sciences, College of Liberal Arts and Sciences, University of Westminster, 115 New Cavendish Street, London W1W 6UW, UK
4
Department of Histopathology, Kingston Hospital, Galsworthy Road, London KT2 7QE, UK
*
Author to whom correspondence should be addressed.
Biology 2020, 9(3), 52; https://doi.org/10.3390/biology9030052
Received: 15 January 2020 / Revised: 24 February 2020 / Accepted: 6 March 2020 / Published: 9 March 2020
Prostate cancer (PCa) is the second-leading cause of cancer-related death among men. microRNAs have been identified as having potential roles in tumorigenesis. An oncomir, miR-21, is commonly highly upregulated in many cancers, including PCa, and showed correlation with the Wnt-signaling axis to increase invasion. Wnt-11 is a developmentally regulated gene and has been found to be upregulated in PCa, but its mechanism is unknown. The present study aimed to investigate the roles of miR-21 and Wnt-11 in PCa in vivo and in vitro. First, different Gleason score PCa tissue samples were used; both miR-21 and Wnt-11 expressions correlate with high Gleason scores in PCa patient tissues. This data then was confirmed with formalin-fixed paraffin cell blocks using PCa cell lines LNCaP and PC3. Cell survival and colony formation studies proved that miR-21 involves in cells’ behaviors, as well as the epithelial-mesenchymal transition. Consistent with the previous data, silencing miR-21 led to significant inhibition of cellular invasiveness. Overall, these results suggest that miR-21 plays a significant role related to Wnt-11 in the pathophysiology of PCa.
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Keywords:
microRNA; in situ hybridization; prostate cancer; Wnt-11; miR-21
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MDPI and ACS Style
Arisan, E.D.; Rencuzogullari, O.; Freitas, I.L.; Radzali, S.; Keskin, B.; Kothari, A.; Warford, A.; Uysal-Onganer, P. Upregulated Wnt-11 and miR-21 Expression Trigger Epithelial Mesenchymal Transition in Aggressive Prostate Cancer Cells. Biology 2020, 9, 52.
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