Contributions of Chondroitin Sulfate, Keratan Sulfate and N-linked Oligosaccharides to Inhibition of Neurite Outgrowth by Aggrecan
Abstract
1. Introduction
2. Materials and Methods
2.1. Preparation of Aggrecan
2.2. Enzyme Digestions
2.3. ELISA Assay
2.4. Neurite Outgrowth Assay
2.5. Image Acquisition
2.6. Data Analysis
3. Results
3.1. NS-1 Cells Extend Neurites in a Dose-Dependent Fashion When Exposed to Varying Amounts of NGF
3.2. ELISA Assay Confirms Adsorption to Wells and Reveals Greater Access to G1-Domain Following Enzymatic Digestion
3.3. NS-1 Cell Neurite Outgrowth is Inhibited in a Concentration-Dependent Manner by Bound Aggrecan
3.4. Quantification of Inhibition of NGF-Stimulated Outgrowth Due to Aggrecan
4. Discussion
5. Conclusions
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Hering, T.M.; Beller, J.A.; Calulot, C.M.; Snow, D.M. Contributions of Chondroitin Sulfate, Keratan Sulfate and N-linked Oligosaccharides to Inhibition of Neurite Outgrowth by Aggrecan. Biology 2020, 9, 29. https://doi.org/10.3390/biology9020029
Hering TM, Beller JA, Calulot CM, Snow DM. Contributions of Chondroitin Sulfate, Keratan Sulfate and N-linked Oligosaccharides to Inhibition of Neurite Outgrowth by Aggrecan. Biology. 2020; 9(2):29. https://doi.org/10.3390/biology9020029
Chicago/Turabian StyleHering, Thomas M., Justin A. Beller, Christopher M. Calulot, and Diane M. Snow. 2020. "Contributions of Chondroitin Sulfate, Keratan Sulfate and N-linked Oligosaccharides to Inhibition of Neurite Outgrowth by Aggrecan" Biology 9, no. 2: 29. https://doi.org/10.3390/biology9020029
APA StyleHering, T. M., Beller, J. A., Calulot, C. M., & Snow, D. M. (2020). Contributions of Chondroitin Sulfate, Keratan Sulfate and N-linked Oligosaccharides to Inhibition of Neurite Outgrowth by Aggrecan. Biology, 9(2), 29. https://doi.org/10.3390/biology9020029