Next Article in Journal
Sources and Fates of Carbamyl Phosphate: A Labile Energy-Rich Molecule with Multiple Facets
Previous Article in Journal
Inpactor, Integrated and Parallel Analyzer and Classifier of LTR Retrotransposons and Its Application for Pineapple LTR Retrotransposons Diversity and Dynamics
Article Menu

Export Article

Open AccessArticle
Biology 2018, 7(2), 33; https://doi.org/10.3390/biology7020033

The Effect of Overexpressed DdRabS on Development, Cell Death, Vesicular Trafficking, and the Secretion of Lysosomal Glycosidase Enzymes

1
Biology Department, University of Arkansas at Little Rock, 2801 South University Ave, Little Rock, AR 72204-1099, USA
2
Otsuka Pharmaceuticals, Greater Pittsburg Area, Pittsburgh, PA 15201, USA
3
Faculty Member of Biology Department, Baghdad University, Baghdad 10071, Iraq
*
Author to whom correspondence should be addressed.
Received: 4 April 2018 / Revised: 11 May 2018 / Accepted: 21 May 2018 / Published: 28 May 2018
Full-Text   |   PDF [3116 KB, uploaded 28 May 2018]   |  

Abstract

Rab GTPases are essential regulators of many cellular processes and play an important role in downstream signaling vital to proper cell function. We sought to elucidate the role of novel D. discoideum GTPase RabS. Cell lines over-expressing DdRabS and expressing DdRabS N137I (dominant negative (DN)) proteins were generated, and it was determined that DdRabS localized to endosomes, ER-Golgi membranes, and the contractile vacuole system. It appeared to function in vesicular trafficking, and the secretion of lysosomal enzymes. Interestingly, microscopic analysis of GFP-tagged DdRabS (DN) cells showed differential localization to lysosomes and endosomes compared to GFP-tagged DdRabS overexpressing cells. Both cell lines over-secreted lysosomal glycosidase enzymes, especially β-glucosidase. Furthermore, DdRabS overexpressing cells were defective in aggregation due to decreased cell–cell cohesion and sensitivity to cAMP, leading to abnormal chemotactic migration, the inability to complete development, and increased induced cell death. These data support a role for DdRabS in trafficking along the vesicular and biosynthetic pathways. We hypothesize that overexpression of DdRabS may interfere with GTP activation of related proteins essential for normal development resulting in a cascade of defects throughout these processes. View Full-Text
Keywords: Dictyostelium; RabS; vesicular trafficking; lysosomal enzymes; β-glucosidase; GTPase; development; cell death Dictyostelium; RabS; vesicular trafficking; lysosomal enzymes; β-glucosidase; GTPase; development; cell death
Figures

Figure 1

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).
SciFeed

Share & Cite This Article

MDPI and ACS Style

Yarbrough, A.; Maringer, K.; Saheb, E.J.; Jawed, S.; Bush, J. The Effect of Overexpressed DdRabS on Development, Cell Death, Vesicular Trafficking, and the Secretion of Lysosomal Glycosidase Enzymes. Biology 2018, 7, 33.

Show more citation formats Show less citations formats

Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]
Biology EISSN 2079-7737 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top