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Biology 2018, 7(1), 17; https://doi.org/10.3390/biology7010017

Detection and Enhancement of Ketocarotenoid Accumulation in the Newly Isolated Sarcinoid Green Microalga Chlorosarcinopsis PY02

1
Department of Biology, Faculty of Science, Silpakorn University, Nakhonpathom 73000, Thailand
2
School of Biological Sciences, Royal Holloway University of London, Egham TW20 0EX, UK
3
Institute of Structural and Molecular Biology, University College London, London WC1E 6BT, UK
*
Author to whom correspondence should be addressed.
Received: 19 December 2017 / Revised: 2 February 2018 / Accepted: 7 February 2018 / Published: 12 February 2018
(This article belongs to the Special Issue Microalgal Biotechnology)
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Abstract

The sarcinoid alga PY02 is a newly isolated soil alga native to western Thailand. In this study PY02 is described, the carotenoid profile of the green and red forms of the algal cells are compared, and the effect of nitrogen reduction and media volume on ketocarotenoid production are reported. Partial sequences of the genes from elongation factor Tu (tufA) and 18S rRNA reveal that the alga is from the Chlorosarcinopsis genus. Growth studies demonstrated that Chlorosarcinopsis PY02 is capable of photoautotrophic, heterotrophic and mixotrophic growth. A gradual change in colony colour from green to red was observed over a period of four weeks under mixotrophic conditions. Pigment analysis of lyophilized red cells using ultrahigh performance liquid chromatography (UPLC) with Photo Diode Array Detection (PDA), showed for the first time that an alga from the genus Chlorosarcinopsis is capable of producing ketocarotenoids such as adonixanthin and 3-OH-echinenone, with canthaxanthin as the dominant pigment. Interestingly, a reduction of nitrogen in the medium exerts a positive effect on the rate of colour change from one month to less than seven days. Enhancements of the canthaxanthin content from 520 to 1504 or 1427 µg·gDW−1 were detected under 50% and 10% nitrogen content, respectively. An increase of 16% in biomass production of PY02 was unexpectedly detected from a 50% nitrogen reduction under mixotrophic culture. Notably, in liquid mixotrophic media with volumes of 15, 30 and 60 mL, the lowest volume produced a significantly higher biomass and canthaxanthin content. View Full-Text
Keywords: natural colourant; carotenoid; ketocarotenoid; canthaxanthin; nitrogen deprivation; Chlorosarcinopsis natural colourant; carotenoid; ketocarotenoid; canthaxanthin; nitrogen deprivation; Chlorosarcinopsis
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Cherdchukeattisak, P.; Fraser, P.D.; Purton, S.; Brocklehurst, T.W. Detection and Enhancement of Ketocarotenoid Accumulation in the Newly Isolated Sarcinoid Green Microalga Chlorosarcinopsis PY02. Biology 2018, 7, 17.

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