Next Article in Journal
Modulation of Group I Ribozyme Activity by Cationic Porphyrins
Next Article in Special Issue
NPPD: A Protein-Protein Docking Scoring Function Based on Dyadic Differences in Networks of Hydrophobic and Hydrophilic Amino Acid Residues
Previous Article in Journal
MALDI Mass Spectrometry Imaging of Lipids and Gene Expression Reveals Differences in Fatty Acid Metabolism between Follicular Compartments in Porcine Ovaries
Previous Article in Special Issue
Easy and Rapid Binding Assay for Functional Analysis of Disulfide-Containing Peptides by a Pull-Down Method Using a Puromycin-Linker and a Cell-Free Translation System
Open AccessReview

Recent Advances in the Analysis of Macromolecular Interactions Using the Matrix-Free Method of Sedimentation in the Analytical Ultracentrifuge

National Centre for Macromolecular Hydrodynamics, University of Nottingham, Sutton Bonington LE12 5RD, UK
Department of Food Engineering, Abant Izzet Baysal University, Bolu 14280, Turkey
Faculty of Medicine and Health Sciences, University of Nottingham, Clifton Boulevard, Nottingham NG7 2RD, UK
Author to whom correspondence should be addressed.
Academic Editor: Thorsten Berg
Biology 2015, 4(1), 237-250;
Received: 1 December 2014 / Revised: 8 February 2015 / Accepted: 16 February 2015 / Published: 6 March 2015
(This article belongs to the Special Issue Protein-Protein Interactions)
Sedimentation in the analytical ultracentrifuge is a matrix free solution technique with no immobilisation, columns, or membranes required and can be used to study self-association and complex or “hetero”-interactions, stoichiometry, reversibility and interaction strength of a wide variety of macromolecular types and across a very large dynamic range (dissociation constants from 10−12 M to 10−1 M). We extend an earlier review specifically highlighting advances in sedimentation velocity and sedimentation equilibrium in the analytical ultracentrifuge applied to protein interactions and mucoadhesion and to review recent applications in protein self-association (tetanus toxoid, agrin), protein-like carbohydrate association (aminocelluloses), carbohydrate-protein interactions (polysaccharide-gliadin), nucleic-acid protein (G-duplexes), nucleic acid-carbohydrate (DNA-chitosan) and finally carbohydrate-carbohydrate (xanthan-chitosan and a ternary polysaccharide complex) interactions. View Full-Text
Keywords: protein; carbohydrate; nucleic acid; interaction; hydrodynamics protein; carbohydrate; nucleic acid; interaction; hydrodynamics
Show Figures

Figure 1

MDPI and ACS Style

Harding, S.E.; Gillis, R.B.; Almutairi, F.; Erten, T.; Kök, M.Ş.; Adams, G.G. Recent Advances in the Analysis of Macromolecular Interactions Using the Matrix-Free Method of Sedimentation in the Analytical Ultracentrifuge. Biology 2015, 4, 237-250.

Show more citation formats Show less citations formats

Article Access Map by Country/Region

Only visits after 24 November 2015 are recorded.
Back to TopTop