Next Article in Journal
Acknowledgement to Reviewers of Biology in 2013
Next Article in Special Issue
Screening for Antifibrotic Compounds Using High Throughput System Based on Fluorescence Polarization
Previous Article in Journal
McArdle Disease and Exercise Physiology
Previous Article in Special Issue
Miniaturized Bioaffinity Assessment Coupled to Mass Spectrometry for Guided Purification of Bioactives from Toad and Cone Snail
Article Menu

Export Article

Open AccessArticle
Biology 2014, 3(1), 167-175;

In Vitro High Throughput Screening, What Next? Lessons from the Screening for Aurora Kinase Inhibitors

INSERM UJF U823 Institut Albert Bonniot, Team 4: Chromatin and Epigenetic, BP 170, 38 042 Grenoble Cedex 9, France
Faculty of Biology, VNU University of Science, HaNoi 10 000, Vietnam
Department of biology, University of Natural Sciences, Ho Chi Minh 70 000, Vietnam
Department of biology, University of Technology, DaNang 55 000, Vietnam
UMR 176 CNRS-Institut Curie, Bat 110 Centre Universitaire, Orsay 91405, France
Author to whom correspondence should be addressed.
Received: 3 December 2013 / Revised: 13 February 2014 / Accepted: 14 February 2014 / Published: 27 February 2014
(This article belongs to the Special Issue Screening for Biologically Active Compounds)
Full-Text   |   PDF [387 KB, uploaded 27 February 2014]   |  


Based on in vitro assays, we performed a High Throughput Screening (HTS) to identify kinase inhibitors among 10,000 small chemical compounds. In this didactic paper, we describe step-by-step the approach to validate the hits as well as the major pitfalls encountered in the development of active molecules. We propose a decision tree that could be adapted to most in vitro HTS. View Full-Text
Keywords: aurora; kinase; kinase inhibitor; Gck; Nuak1; Tak-1 aurora; kinase; kinase inhibitor; Gck; Nuak1; Tak-1

Figure 1

This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

Share & Cite This Article

MDPI and ACS Style

Hoang, T.-M.-N.; Vu, H.-L.; Le, L.-T.-T.; Nguyen, C.-H.; Molla, A. In Vitro High Throughput Screening, What Next? Lessons from the Screening for Aurora Kinase Inhibitors. Biology 2014, 3, 167-175.

Show more citation formats Show less citations formats

Related Articles

Article Metrics

Article Access Statistics



[Return to top]
Biology EISSN 2079-7737 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top