Transdermal Semaglutide Administration in Mice: Reduces Body Weight by Suppressing Appetite and Enhancing Metabolic Rate
Simple Summary
Abstract
1. Introduction
2. Materials and Methods
2.1. Animals and Treatment
2.2. Body Mass
2.3. Blood Indexes
2.4. Metabolic Indexes
2.5. Behavioral Measurements
2.6. Body Fat Deposit and Organs Collection
2.7. Real-Time RT-qPCR Analysis
3. Data Analysis
4. Results
4.1. Body Weight and Fat Distribution
4.2. Blood Indexes
4.3. Metabolic Rate
4.4. Organ Weight
4.5. Expression of Feeding-Related Neuropeptides
4.6. Anxiety-like and Exploratory Behaviors
4.7. Hematoxylin-Eosin Staining Pathological Section
5. Discussion
5.1. Transdermal Semaglutide Delivery Is as Effective as Injection Treatment for Reducing Body Weights
5.2. Transdermal Semaglutide Delivery Is More Potent than the Injection Treatment
5.3. Transdermal Drug Delivery Has Good Security in Obesity Treatment
6. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Gene | Sequence 5′-3′ | Size (bp) |
---|---|---|
NPY-f | TCGTGTGTTTGGGCATTCTG | 128 |
NPY-r | TCTGGTGATGAGATTGATGTAGTG | |
CART-f | ACGAGAAGGAGCTGCCAAG | 153 |
CART-r | GCTCTCCAGCGTCACACAT | |
POMC-f | GAAGATGCCGAGATTCTGCT | 175 |
POMC-r | CTCCAGCGAGAGGTCGAGTT | |
AgRP-f | ACCTTAGGGAGGCACCTCAT | 151 |
AgRP-r | AGCAACATTGCAGTCAGCAT | |
ObRb-f | TAAAGCTCTCGTGGCGCTCT | 195 |
ObRb-r | TCCACACGAGCAAGAACAAC | |
β-Actin-f | CGTAAAGACCTCTATGCCAA | 317 |
β-Actin-r | GCGCAAGTTAGGTTTTGTC |
Organs | Control | Injection | TDDS | p |
---|---|---|---|---|
Heart | 0.169 ± 0.014 | 0.148 ± 0.006 | 0.1712 ± 0.008 | ns |
Liver | 1.236 ± 0.036 b | 1.0942 ± 0.018 a | 1.201 ± 0.042 b | * |
Spleen | 0.072 ± 0.006 | 0.062 ± 0.003 | 0.062 ± 0.003 | ns |
Lung | 0.149 ± 0.009 a | 0.161 ± 0.007 b | 0.182 ± 0.007 c | * |
Kidney | 0.343 ± 0.012 | 0.329 ± 0.011 | 0.312 ± 0.006 | ns |
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Li, W.; Cai, R.; Yin, B.; Zhou, Y.; Dong, X.; Li, W.; Wen, J. Transdermal Semaglutide Administration in Mice: Reduces Body Weight by Suppressing Appetite and Enhancing Metabolic Rate. Biology 2025, 14, 575. https://doi.org/10.3390/biology14050575
Li W, Cai R, Yin B, Zhou Y, Dong X, Li W, Wen J. Transdermal Semaglutide Administration in Mice: Reduces Body Weight by Suppressing Appetite and Enhancing Metabolic Rate. Biology. 2025; 14(5):575. https://doi.org/10.3390/biology14050575
Chicago/Turabian StyleLi, Wenjing, Ruilin Cai, Binxin Yin, Yingying Zhou, Xinyuan Dong, Wenting Li, and Jing Wen. 2025. "Transdermal Semaglutide Administration in Mice: Reduces Body Weight by Suppressing Appetite and Enhancing Metabolic Rate" Biology 14, no. 5: 575. https://doi.org/10.3390/biology14050575
APA StyleLi, W., Cai, R., Yin, B., Zhou, Y., Dong, X., Li, W., & Wen, J. (2025). Transdermal Semaglutide Administration in Mice: Reduces Body Weight by Suppressing Appetite and Enhancing Metabolic Rate. Biology, 14(5), 575. https://doi.org/10.3390/biology14050575