N-WASP Attenuates Cell Proliferation and Migration through ERK2-Dependent Enhanced Expression of TXNIP
Abstract
:Simple Summary
Abstract
1. Introduction
2. Materials and Methods
2.1. Cell Culture and Generation of Stable Sublines by Lentiviral Transduction
2.2. Cell Proliferation Assay
2.3. Wound Healing Assay
2.4. Western Blot Analysis
2.5. Immunofluorescence
2.6. KinexTM Antibody Microarray Analysis
2.7. Real-Time PCR
2.8. Statistical Analysis
3. Results
3.1. Expression of N-WASP Is Reduced in SCC Compared to Matched Perilesional Controls
3.2. Elevated Expression of N-WASP in HSC-5 Cells Reduced Cell Proliferation and Cell Migration
3.3. FOXO1 Expression Is Reduced in HSC-5NW Cells Compared to HSC-5CN Cells
3.4. Inhibition of ERK2 in HSC-5NW Cells Enhanced Cell Proliferation, Nuclear Localization of FOXO1 and Cell Migration
3.5. Knockdown of TXNIP in HSC-5NW Cells Restored Cell Proliferation and Migration
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Chung, Y.J.; Salvi, A.; Kalailingam, P.; Alnawaz, M.; Tan, S.H.; Pan, J.Y.; Tan, N.S.; Thanabalu, T. N-WASP Attenuates Cell Proliferation and Migration through ERK2-Dependent Enhanced Expression of TXNIP. Biology 2022, 11, 582. https://doi.org/10.3390/biology11040582
Chung YJ, Salvi A, Kalailingam P, Alnawaz M, Tan SH, Pan JY, Tan NS, Thanabalu T. N-WASP Attenuates Cell Proliferation and Migration through ERK2-Dependent Enhanced Expression of TXNIP. Biology. 2022; 11(4):582. https://doi.org/10.3390/biology11040582
Chicago/Turabian StyleChung, Yat Joong, Amrita Salvi, Pazhanichamy Kalailingam, Myra Alnawaz, Suat Hoon Tan, Jiun Yit Pan, Nguan Soon Tan, and Thirumaran Thanabalu. 2022. "N-WASP Attenuates Cell Proliferation and Migration through ERK2-Dependent Enhanced Expression of TXNIP" Biology 11, no. 4: 582. https://doi.org/10.3390/biology11040582
APA StyleChung, Y. J., Salvi, A., Kalailingam, P., Alnawaz, M., Tan, S. H., Pan, J. Y., Tan, N. S., & Thanabalu, T. (2022). N-WASP Attenuates Cell Proliferation and Migration through ERK2-Dependent Enhanced Expression of TXNIP. Biology, 11(4), 582. https://doi.org/10.3390/biology11040582