Sexual Dimorphism in Extracellular Matrix Composition and Viscoelasticity of the Healthy and Inflamed Mouse Brain
Abstract
:Simple Summary
Abstract
1. Introduction
2. Materials and Methods
2.1. Animals and EAE Model
2.2. MRE Acquisition
2.3. Data Reconstruction
2.4. Tissue Processing
2.5. Gene Expression Analysis
2.6. Histology
2.7. Statistical Analysis
3. Results
3.1. Sex-Specific Viscoelastic Properties of Healthy Mouse Brain
3.2. Differences in Extracellular Matrix Composition in the Healthy Brain of Males and Females
3.3. Sex-Specific Changes of Brain Viscoelastic Properties during EAE
3.4. Extracellular Matrix Remodeling in Female and Male EAE Brains
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Cortex | Hippocampus | |||||
---|---|---|---|---|---|---|
Gene | EAE/Naive | EAE/Naive | EAE / | EAE/Naive | EAE/Naive | EAE / |
Lama5 | 2.91 | 3.04 | −3.85 | 1.83 | −1.08 | −2.42 |
Lama4 | 3.10 | 2.70 | −3.86 | 1.08 | 1.11 | −1.69 |
Col4a1 | 2.99 | 2.01 | −2.38 | −1.51 | −1.37 | −1.12 |
Col1a1 | −1.29 | −2.12 | −1.34 | −2.07 | −1.33 | 1.26 |
Fn1 | 1.12 | −1.43 | 1.18 | −1.19 | 1.14 | 1.24 |
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Batzdorf, C.S.; Morr, A.S.; Bertalan, G.; Sack, I.; Silva, R.V.; Infante-Duarte, C. Sexual Dimorphism in Extracellular Matrix Composition and Viscoelasticity of the Healthy and Inflamed Mouse Brain. Biology 2022, 11, 230. https://doi.org/10.3390/biology11020230
Batzdorf CS, Morr AS, Bertalan G, Sack I, Silva RV, Infante-Duarte C. Sexual Dimorphism in Extracellular Matrix Composition and Viscoelasticity of the Healthy and Inflamed Mouse Brain. Biology. 2022; 11(2):230. https://doi.org/10.3390/biology11020230
Chicago/Turabian StyleBatzdorf, Clara Sophie, Anna Sophie Morr, Gergely Bertalan, Ingolf Sack, Rafaela Vieira Silva, and Carmen Infante-Duarte. 2022. "Sexual Dimorphism in Extracellular Matrix Composition and Viscoelasticity of the Healthy and Inflamed Mouse Brain" Biology 11, no. 2: 230. https://doi.org/10.3390/biology11020230