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Article

Nitric-Oxide-Mediated Vasodilation of Bioactive Compounds Isolated from Hypericum revolutum in Rat Aorta

1
Department of Natural Products and Alternative Medicine, Faculty of Pharmacy, King Abdulaziz University, Jeddah 21589, Saudi Arabia
2
Department of Pharmacognosy, Faculty of Pharmacy, Cairo University, Cairo 11562, Egypt
3
Batterjee Medical College, North Obhur, Abdullah Al-Faisal Street, Jeddah 21442, Saudi Arabia
4
Department of Pharmacognosy, Faculty of Pharmacy, Assiut University, Assiut 71526, Egypt
5
Department of Pharmaceutical Chemistry, College of Pharmacy, King Abdulaziz University, Jeddah 21589, Saudi Arabia
6
Department of Pharmacology, Faculty of Pharmacy, Zagazig University, Zagazig 44519, Egypt
*
Author to whom correspondence should be addressed.
Academic Editor: Guo-Chang Fan
Biology 2021, 10(6), 541; https://doi.org/10.3390/biology10060541
Received: 17 May 2021 / Revised: 4 June 2021 / Accepted: 15 June 2021 / Published: 17 June 2021
Hypericum revolutum (HR) is reported to produce vasodilating activity in phenylephrine-precontracted aortae, where the chloroform fraction is the most potent. Chemical investigation of this fraction yielded two new compounds, revolutin (1) and hyperevolutin C (2), along with three known metabolites, β-sitosterol (3), euxanthone (4), and 2,3,4-tirmethoxy xanthone (5). Isolated compounds 1, 2, 3, and 5 produce vasodilation activities that are dependent on endothelial nitric oxide release.
Vasodilators are an important class in the management of hypertension and related cardiovascular disorders. In this regard, the chloroform fraction of Hypericum revolutum (HR) has been reported to produce vasodilating activity in phenylephrine-precontracted aortae. The current work aims to identify the active metabolites in the chloroform fraction of HR and illustrate the possible mechanism of action. The vasodilation activities were investigated using the isolated artery technique. NO vascular release was assessed by utilizing the NO-sensitive fluorescent probe DAF-FM. Free radical scavenging capacity was assessed utilizing DPPH. Chemical investigation of this fraction yielded two new compounds, revolutin (1) and hyperevolutin C (2), along with three known metabolites, β-sitosterol (3), euxanthone (4), and 2,3,4-tirmethoxy xanthone (5). Compounds 1, 2, 3, and 5 showed significant vasodilation activities that were blocked by either endothelial denudation or L-NAME (nitric oxide synthase inhibitor), pointing towards a role of endothelial nitric oxide in their activities. In confirmation of this role, compounds 13 showed a significant release of NO from isolated vessels, as indicated by DAF-FM. On the other hand, only compound 5 showed free radical scavenging activities, as indicated by DPPH. In conclusion, isolated compounds 1, 2, 3, and 5 produce vasodilation activities that are dependent on endothelial nitric oxide release. View Full-Text
Keywords: Hypericum revolutum; vasodilation; nitric oxide; xanthones; phloroglucinol Hypericum revolutum; vasodilation; nitric oxide; xanthones; phloroglucinol
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MDPI and ACS Style

Abdallah, H.M.; Timraz, N.Z.; Ibrahim, S.R.M.; El-Halawany, A.M.; Malebari, A.M.; Shehata, I.A.; El-Bassossy, H.M. Nitric-Oxide-Mediated Vasodilation of Bioactive Compounds Isolated from Hypericum revolutum in Rat Aorta. Biology 2021, 10, 541. https://doi.org/10.3390/biology10060541

AMA Style

Abdallah HM, Timraz NZ, Ibrahim SRM, El-Halawany AM, Malebari AM, Shehata IA, El-Bassossy HM. Nitric-Oxide-Mediated Vasodilation of Bioactive Compounds Isolated from Hypericum revolutum in Rat Aorta. Biology. 2021; 10(6):541. https://doi.org/10.3390/biology10060541

Chicago/Turabian Style

Abdallah, Hossam M., Noha Z. Timraz, Sabrin R. M. Ibrahim, Ali M. El-Halawany, Azizah M. Malebari, Ibrahim A. Shehata, and Hany M. El-Bassossy. 2021. "Nitric-Oxide-Mediated Vasodilation of Bioactive Compounds Isolated from Hypericum revolutum in Rat Aorta" Biology 10, no. 6: 541. https://doi.org/10.3390/biology10060541

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