Hair Testing for Classic Drugs of Abuse to Monitor Cocaine Use Disorder in Patients Following Transcranial Magnetic Stimulation Protocol Treatment
Abstract
:Simple Summary
Abstract
1. Introduction
2. Materials and Methods
2.1. Chemicals and Reagents
2.2. Study Design
2.3. Preparation of Calibration Standards and Quality Control Samples
2.4. Sample Preparation for Analysis of COC, BZE, MOR, 6-MAM, COD, MTD, and EDDP in Hair Samples
2.5. Sample Preparation for Analysis A, MA, MDA, MDMA in Hair Samples
2.6. Sample Preparation for Analysis of THC and THC-COOH
2.7. GC–MS Instrumentation
2.8. Validation of Analytical Method
3. Results
3.1. Validation Results
3.2. Sample Results
4. Discussion
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Compounds | GC/MS | ||
---|---|---|---|
Rt | m/z ions | m/z ions deuterated IS | |
6 _MAM-TMS | 7.9 | 287,340,399 | 290,343,402 |
MOR-TMS | 7.0 | 401,414,429 | 404,417,432 |
COD-TMS | 6.7 | 196,234,371 | 316,346,374 |
COC | 6,0 | 82,182,303 | 85,185,306 |
BZE-TMS | 6,2 | 82,240,361 | 85,243,364 |
MTD | 5,2 | 72,223,294 | 81,233,303 |
EDDP | 4,6 | 220,262,277 | 223,265,280 |
AP-TFA | 4.8 | 91,118,140 | 97,124,146 |
MA-TFA | 5.0 | 91,118,154 | 96,123,159 |
MDMA-TFA | 5.8 | 135,154,162 | 140,159,167 |
MDA-TFA | 6.0 | 135,162,275 | 140,167,280 |
THC-TMS | 11.5 | 303,371,386 | 306,374,389 |
THC-COOH-TMS | 9.5 | 371,473,488 | 374,476,491 |
Compounds | Slope; mean ± SD a | Intercept mean ± SD a | Correlation Coefficient (r2) mean ± SD a | LOD ng/mg | LOQ ng/mg |
---|---|---|---|---|---|
6 _MAM-TMS | 0.010 ± 0.021 | 0.195 ± 0.003 | 0.998 ± 0.002 | 0.05 | 0.1 |
MOR-TMS | 0.021 ± 0.032 | 0.064 ± 0.001 | 0.998 ± 0.003 | 0.05 | 0.1 |
COD-TMS | 0,0064 ± 0.011 | 0.021 ± 0.001 | 0.997 ± 0.002 | 0.05 | 0.1 |
COC | 0.0071 ± 0.011 | 0.083 ± 0.005 | 0.991 ± 0.001 | 0.05 | 0.1 |
BZE-TMS | 0.0045 ± 0.042 | 0.091 ± 0.002 | 0.996 ± 0.002 | 0.05 | 0.1 |
MTD | 0.0058 ± 0.010 | 0.064 ± 0.001 | 0.998 ± 0.002 | 0.05 | 0.1 |
EDDP | 0.186 ± 0.021 | 0.016 ± 0.002 | 0.994 ± 0.004 | 0.05 | 0.1 |
AP-TFA | 0.022 ± 0.001 | 0.413 ± 0.004 | 0.990 ± 0.004 | 0.05 | 0.1 |
MA-TFA | 0.037 ± 0.002 | 1.141 ± 0.001 | 0.993 ± 0.002 | 0.05 | 0.1 |
MDMA-TFA | 0.037 ± 0.002 | 2.323 ± 0.001 | 0.998 ± 0.001 | 0.05 | 0.1 |
MDA-TFA | 0.0044 ± 0.011 | 0.434 ± 0.002 | 0.991 ± 0.005 | 0.05 | 0.1 |
THC-TMS | 0.011 ± 0.010 | 0.021 ± 0.005 | 0.999 ± 0.004 | 0.03 | 0.1 |
THC-COOH-TMS | 0.062 ± 0.021 | 0.028 ± 0.001 | 0.998 ± 0.001 | 0.02 | 0.05 |
Compounds | Intra-Day Imprecision (CV%) in Quality Control Hair Samples | Intra-Day Inaccuracy (Error%) in Quality Control Hair Samples | Inter-Day Imprecision (CV%) in Quality Control Hair Samples | Inter-Day Inaccuracy (Error%) in Quality Control Hair Samples | ||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
Low QC | Medium QC | High QC | Low QC | Medium QC | High QC | Low QC | Medium QC | High QC | Low QC | Medium QC | High QC | |
6-MAM-TMS | 3.4 | 6.8 | 5.3 | 5.8 | 7.2 | 4.3 | 3.2 | 4.3 | 5.7 | 3.8 | 4.7 | 7.3 |
MOR-TMS | 5.1 | 4.6 | 8.1 | 2.8 | 5.5 | 6.8 | 4.3 | 5.7 | 6.1 | 2.4 | 5.8 | 3.4 |
COD-TMS | 5.5 | 3.8 | 4.5 | 6.6 | 2.8 | 4.8 | 5.9 | 4.1 | 3.3 | 6.8 | 9.1 | 4.2 |
COC | 3.1 | 4.2 | 5.8 | 5.5 | 4.3 | 6.6 | 4.7 | 3.2 | 2.8 | 5.9 | 6.6 | 6.1 |
BZE-TMS | 5.8 | 2.3 | 4.1 | 3.3 | 5.2 | 4.1 | 6.6 | 5.4 | 7.2 | 8.0 | 5.6 | 6.6 |
MTD | 4.4 | 3.2 | 5.8 | 2.5 | 7.2 | 4.1 | 6.7 | 8.2 | 2.1 | 4.9 | 5.2 | 7.1 |
EDDP | 2.2 | 5.1 | 4.3 | 4.8 | 2.4 | 6.6 | 5.9 | 2.5 | 4.3 | 9.1 | 5.7 | 8.3 |
AP-TFA | 6.8 | 3.4 | 5.3 | 6.1 | 6.5 | 7.4 | 8.1 | 4.3 | 2.8 | 4.7 | 5.2 | 2.8 |
MA-TFA | 5.1 | 4.5 | 8.1 | 6.6 | 4.8 | 6.4 | 7.2 | 4.2 | 7.2 | 6.8 | 5.5 | 6.7 |
MDMA-TFA | 3.3 | 6.3 | 4.2 | 8.1 | 7.1 | 5.5 | 4.6 | 8.6 | 5.3 | 7.3 | 8.5 | 2.5 |
MDA-TFA | 4.2 | 2.5 | 5.3 | 6.6 | 8.2 | 4.2 | 5.3 | 4.9 | 8.1 | 2.4 | 5.2 | 6.3 |
THC-TMS | 5.3 | 4.2 | 2.6 | 4.4 | 3.2 | 7.2 | 5.3 | 4.8 | 5.1 | 6.6 | 8.1 | 4.3 |
THC-COOH-TMS | 2.2 | 3.2 | 6.3 | 5.4 | 8.2 | 4.6 | 6.2 | 5.1 | 7.1 | 5.3 | 4.3 | 2.1 |
Patient | Days in treatment at sampling | Last month cocaine frequency level at baseline 1 | Days from last use reported | Proportion of in treatment cocaine-positive urine screen (Pos/Tot) | COC (Dis/Prox) (ng/mg) | BEG (Dis/Prox) (ng/mg) | Level of consumption based on hair analysis at baseline 2 | Level of consumption based on hair analysis at sampling 2 |
---|---|---|---|---|---|---|---|---|
Case 1 | 77 | Abstinence | 158 | 0/10 | 4.10/3.10 | 0.60/0.40 | Medium positivity | Low positivity |
Case 2 | 219 | Low frequency | 176 | 7/25 | 4.50/3.60 | 1.90/1.60 | Medium positivity | Low positivity |
Case 3 | 39 | High frequency | 0 | 2/6 | 85.10/46.30 | 20.70/10.70 | High positivity | High positivity |
Case 4 | 1 | Low frequency | 1 | 1/1 | 58.50 * | 12.90 * | High positivity * | High positivity * |
Case 5 | 92 | Low frequency | 99 | 0/14 | 16.70/0.50 | 0.60/0.40 | Medium positivity | Low positivity |
Case 6 | 210 | High frequency | 280 | 0/27 | 1.20/Neg | 2.10/Neg | Low positivity | Absence |
Case 7 | 166 | Abstinence | 413 | 0/10 | 0.70/Neg | 0.50/Neg | Absence | Absence |
Case 8 | 142 | Abstinence | 232 | 0/16 | 1.10/Neg | 2.20/Neg | Low positivity | Absence |
Case 9 | 164 | High frequency | 165 | 0/14 | 2.07/Neg | 1.06/Neg | Low positivity | Absence |
Patient | THC (Dis/Prox) (ng/mg) | THCCOOH (Dis/Prox) (ng/mg) |
---|---|---|
Case 6 | Neg/2.8 | Neg/0.002 |
Case 7 | Neg/0.2 | Neg/0.002 |
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Rotolo, M.C.; Pacifici, R.; Pellegrini, M.; Cardullo, S.; Pérez, L.J.G.; Cuppone, D.; Gallimberti, L.; Madeo, G. Hair Testing for Classic Drugs of Abuse to Monitor Cocaine Use Disorder in Patients Following Transcranial Magnetic Stimulation Protocol Treatment. Biology 2021, 10, 403. https://doi.org/10.3390/biology10050403
Rotolo MC, Pacifici R, Pellegrini M, Cardullo S, Pérez LJG, Cuppone D, Gallimberti L, Madeo G. Hair Testing for Classic Drugs of Abuse to Monitor Cocaine Use Disorder in Patients Following Transcranial Magnetic Stimulation Protocol Treatment. Biology. 2021; 10(5):403. https://doi.org/10.3390/biology10050403
Chicago/Turabian StyleRotolo, Maria Concetta, Roberta Pacifici, Manuela Pellegrini, Stefano Cardullo, Luis J. Gómez Pérez, Diego Cuppone, Luigi Gallimberti, and Graziella Madeo. 2021. "Hair Testing for Classic Drugs of Abuse to Monitor Cocaine Use Disorder in Patients Following Transcranial Magnetic Stimulation Protocol Treatment" Biology 10, no. 5: 403. https://doi.org/10.3390/biology10050403