Blastocystis sp. Carriage and Irritable Bowel Syndrome: Is the Association Already Established?
Abstract
:Simple Summary
Abstract
1. Introduction
2. Methods
2.1. Statistical and Ethical Issues
2.2. Microbiological Techniques
3. Results
4. Discussion
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
Ethics Approval and Consent to Participate
Appendix A
References
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Healthy Controls (n = 36) | IBS patients (n = 36) | p Value | |
---|---|---|---|
Age, years | 30 (29–38.7) | 43 (29.5–50.5) | 0.094 |
Gender, male | 19 (52.8%) | 16 (44.4%) | 0.479 |
Living in rural environment | 3 (8.3%) | 1 (2.8%) | 0.614 |
Regular contact with animals | 11 (30.6%) | 21 (58.3%) | 0.018 |
Travelling abroad in the last 12 months | 26 (72.2%) | 8 (22.2%) | <0.001 |
Travelling to low or middle income country in the last 12 months | 14 (38.8%) | 1 (2.7%) | <0.001 |
Working in close contact with people | 20 (55.6%) | 10 (27.8%) | 0.017 |
Healthy Controls (n = 36) | IBS Patients (n = 36) | p Value | |
---|---|---|---|
Blastocystis sp. detection (microscopy + PCR) | 13 (36.1%) | 7 (19.4%) | 0.114 |
Blastocystis sp. detection by microscopic examination | 10 (27.8%) | 6 (16.7%) | 0.257 |
Parasite burden (n = 16) * | |||
Low (<1 parasite/high-power field ×400) | 4/10 (40%) | 2/6 (33.3%) | 1 |
Medium (1–5 parasite/high-power field ×400) | 4/10 (40%) | 2/6 (33.3%) | 1 |
High (>5 parasite/high-power field ×400) | 2 (20%) | 2/6 (33.3%) | 0.604 |
Positive Blastocystis sp. PCR | 8 (22.2%) | 3 (8.3%) | 0.101 |
Detection of other intestinal microbial eukaryotes | 1 (2.8%) | 3 (8.3%) | 0.614 |
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Salvador, F.; Lobo, B.; Goterris, L.; Alonso-Cotoner, C.; Santos, J.; Sulleiro, E.; Bailo, B.; Carmena, D.; Sánchez-Montalvá, A.; Bosch-Nicolau, P.; et al. Blastocystis sp. Carriage and Irritable Bowel Syndrome: Is the Association Already Established? Biology 2021, 10, 340. https://doi.org/10.3390/biology10040340
Salvador F, Lobo B, Goterris L, Alonso-Cotoner C, Santos J, Sulleiro E, Bailo B, Carmena D, Sánchez-Montalvá A, Bosch-Nicolau P, et al. Blastocystis sp. Carriage and Irritable Bowel Syndrome: Is the Association Already Established? Biology. 2021; 10(4):340. https://doi.org/10.3390/biology10040340
Chicago/Turabian StyleSalvador, Fernando, Beatriz Lobo, Lidia Goterris, Carmen Alonso-Cotoner, Javier Santos, Elena Sulleiro, Begoña Bailo, David Carmena, Adrián Sánchez-Montalvá, Pau Bosch-Nicolau, and et al. 2021. "Blastocystis sp. Carriage and Irritable Bowel Syndrome: Is the Association Already Established?" Biology 10, no. 4: 340. https://doi.org/10.3390/biology10040340
APA StyleSalvador, F., Lobo, B., Goterris, L., Alonso-Cotoner, C., Santos, J., Sulleiro, E., Bailo, B., Carmena, D., Sánchez-Montalvá, A., Bosch-Nicolau, P., Espinosa-Pereiro, J., Fuentes, I., & Molina, I. (2021). Blastocystis sp. Carriage and Irritable Bowel Syndrome: Is the Association Already Established? Biology, 10(4), 340. https://doi.org/10.3390/biology10040340