R-Score: A New Parameter to Assess the Quality of Variants’ Calls Assessed by NGS Using Liquid Biopsies
Abstract
:Simple Summary
Abstract
1. Introduction
2. Materials and Methods
2.1. Patients and Samples
2.2. Laboratory Procedures
2.3. Parameters
2.4. Statistical Analysis
3. Results
3.1. EGFR Mutation Detection by dPCR and NGS-Oncomine
3.2. VAF and MAPD Involvement in the Agreement between dPCR and NGS-Oncomine Calls
3.3. R-Score Is a Useful Parameter to Select Reliable Variant Calls
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Serna-Blasco, R.; Sánchez-Herrero, E.; Berrocal Renedo, M.; Calabuig-Fariñas, S.; Molina-Vila, M.Á.; Provencio, M.; Romero, A. R-Score: A New Parameter to Assess the Quality of Variants’ Calls Assessed by NGS Using Liquid Biopsies. Biology 2021, 10, 954. https://doi.org/10.3390/biology10100954
Serna-Blasco R, Sánchez-Herrero E, Berrocal Renedo M, Calabuig-Fariñas S, Molina-Vila MÁ, Provencio M, Romero A. R-Score: A New Parameter to Assess the Quality of Variants’ Calls Assessed by NGS Using Liquid Biopsies. Biology. 2021; 10(10):954. https://doi.org/10.3390/biology10100954
Chicago/Turabian StyleSerna-Blasco, Roberto, Estela Sánchez-Herrero, María Berrocal Renedo, Silvia Calabuig-Fariñas, Miguel Ángel Molina-Vila, Mariano Provencio, and Atocha Romero. 2021. "R-Score: A New Parameter to Assess the Quality of Variants’ Calls Assessed by NGS Using Liquid Biopsies" Biology 10, no. 10: 954. https://doi.org/10.3390/biology10100954