Coexpression of Fungal Cell Wall-Modifying Enzymes Reveals Their Additive Impact on Arabidopsis Resistance to the Fungal Pathogen, Botrytis cinerea
Abstract
:Simple Summary
Abstract
1. Introduction
2. Materials and Methods
2.1. Arabidopsis Growth Conditions
2.2. Generation of Transgenic Plants
2.3. RNA Extraction, cDNA Synthesis, and Real-Time qPCR
2.4. CW Composition Analysis
2.5. Infection of Arabidopsis Genotypes with Botrytis Cinerea
2.6. Determination of H2O2 Accumulation
2.7. Statistical Analysis
3. Results
3.1. Generation of Transgenic Arabidopsis Plants Expressing A. nidulans Acetylesterases (AnAXE and AnRAE) and Feruloylesterase (AnFAE)
3.2. Expression of Fungal Acetylesterases Reduced the Degree of CW Acetylation in Transgenic Arabidopsis
3.3. Transgenic Arabidopsis Expressing Fungal Feruloylesterase Showed Reduction in CW Feruloylation
3.4. Acetylesterase Expressing Plants Have Increased Resistance to Necrotrophic Fungal Pathogen
3.5. Deacetylation and Deferuloylation of CW Resulted in Expression of Pathogen-Responsive Genes
3.6. Acetylesterase Expressing Plants Have Enhanced H2O2 Accumulation
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Swaminathan, S.; Reem, N.T.; Lionetti, V.; Zabotina, O.A. Coexpression of Fungal Cell Wall-Modifying Enzymes Reveals Their Additive Impact on Arabidopsis Resistance to the Fungal Pathogen, Botrytis cinerea. Biology 2021, 10, 1070. https://doi.org/10.3390/biology10101070
Swaminathan S, Reem NT, Lionetti V, Zabotina OA. Coexpression of Fungal Cell Wall-Modifying Enzymes Reveals Their Additive Impact on Arabidopsis Resistance to the Fungal Pathogen, Botrytis cinerea. Biology. 2021; 10(10):1070. https://doi.org/10.3390/biology10101070
Chicago/Turabian StyleSwaminathan, Sivakumar, Nathan T. Reem, Vincenzo Lionetti, and Olga A. Zabotina. 2021. "Coexpression of Fungal Cell Wall-Modifying Enzymes Reveals Their Additive Impact on Arabidopsis Resistance to the Fungal Pathogen, Botrytis cinerea" Biology 10, no. 10: 1070. https://doi.org/10.3390/biology10101070