Development of a Spinal Cord Injury Model Permissive to Study the Cardiovascular Effects of Rehabilitation Approaches Designed to Induce Neuroplasticity
Abstract
:Simple Summary
Abstract
1. Introduction
2. Materials and Methods
2.1. Ethical Approval
2.2. Experimental Design
2.3. Spinal Cord Injury Surgery
2.4. Post-Surgical Care
2.5. Outcome Surgery
2.6. Ethanasia and Tissue Processing
2.7. Data Analysis
2.8. Immunohistochemistry
2.9. Statistics
3. Results
3.1. Resting Hemodynamics Are Impaired in T3 300 kdyn SCI Rats
3.2. Left Ventricular Systolic Function Is Impaired in T3 300 kdyn SCI Rats
3.3. Moderately-Severe T3 Midline Injury Interrupts Descending Pathways
4. Discussion
4.1. Resting Hemodynamics Are Impaired in T3 300 kdyn SCI Rats
4.2. Left Ventricular Systolic Function Is Impaired in T3 300 kdyn SCI Rats
4.3. T3 Moderately-Severe Contusion Injury Interrupts Descending Pathways
4.4. Comparison to Other Rodent Models of CV Instability
4.5. Limitations
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Naïve | SCI | p-Value | |||||
---|---|---|---|---|---|---|---|
Hemodynamic Data | |||||||
SBP (mmHg) | 121 | ± | 7 | 96 | ± | 11 | <0.001 |
DBP (mmHg) | 70 | ± | 7 | 58 | ± | 9 | <0.001 |
MAP (mmHg) | 88 | ± | 7 | 70 | ± | 9 | <0.001 |
PP (mmHg) | 50 | ± | 4 | 38 | ± | 6 | <0.001 |
HR (BPM) | 413 | ± | 38 | 462 | ± | 48 | 0.008 |
SVR (mmHg·min−1µL−1) * | 0.89 | ± | 0.20 | 0.74 | ± | 0.12 | 0.038 |
Pressure-Volume Data | |||||||
ESV (µL) | 66 | ± | 18 | 59 | ± | 11 | 0.256 |
EDV(µL) | 311 | ± | 47 | 261 | ± | 23 | 0.002 |
Systolic Function | |||||||
SW (mmHg·mL) | 33 | ± | 7 | 21 | ± | 4 | <0.001 |
SWI (mmHg·mL−1100 g−1) | 10.90 | ± | 2.53 | 7.52 | ± | 1.64 | <0.001 |
CO (mL/min) | 102 | ± | 20 | 93 | ± | 12 | 0.201 |
CI (mL·min−1100 g−1) | 33.69 | ± | 7.72 | 35.22 | ± | 7.32 | 0.610 |
SV (µL) | 245 | ± | 33 | 202 | ± | 24 | 0.001 |
Pes (mmHg) | 98 | ± | 11 | 75 | ± | 10 | <0.001 |
EF (%) | 79 | ± | 4 | 77 | ± | 5 | 0.272 |
dP/dtmax (mmHg/s) | 10316 | ± | 809 | 6084 | ± | 755 | <0.001 |
Ees (mmHg/µL) ** | 1.59 | ± | 0.23 | 0.89 | ± | 0.24 | <0.001 |
Ea (mmHg/µL) | 0.41 | ± | 0.09 | 0.38 | ± | 0.07 | 0.296 |
Ea/Ees ** | 0.26 | ± | 0.06 | 0.44 | ± | 0.23 | 0.021 |
PRSW (mmHg) * | 131 | ± | 30 | 94 | ± | 17 | 0.001 |
+dP/dtmax–EDV (mmHg·s−1 µL−1) * | 34 | ± | 7 | 27 | ± | 4 | <0.001 |
Diastolic Function | |||||||
dP/dtmin (mmHg/s) | −5890 | ± | 449 | −4021 | ± | 630 | <0.001 |
Ped (mmHg) | 3 | ± | 2 | 4 | ± | 4 | 0.231 |
τ (ms) | 7.32 | ± | 0.77 | 8.03 | ± | 3 | 0.416 |
Injury Model | ||||||
---|---|---|---|---|---|---|
T3 300 kdyn Contusion | T2 400 kdyn Contusion | T2 200 kdyn Contusion | T2-3 Transection | T4 Complete Crush | T10 400 kdyn Contusion | |
↓ Blood pressure | ✔ | ✔ | ✔ | ✔ | ✖ | ✖ |
↓ Cardiac pressures | ✔ | ✔ | ? | ✔ | ? | ? |
↓ Cardiac output | ✖ | ✔ | ✖ | ✔ | ? | ? |
>15% tissue sparing | ✔ | ✖ | ✖ | ✖ | ? | ? |
>20% white matter Preservation | ✔ | ✖ | ✖ | ✖ | ? | ✖ |
Preserved sub-lesional serotonergic/ catecholaminergic pathways | ✔ | ✖ | ✔ | ? | ✖ | N/A |
References | [24,26,27,34] | [24,34] | [23,30,32] | [25] | [50,51] |
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Wainman, L.; Erskine, E.L.; Ahmadian, M.; Hanna, T.M.; West, C.R. Development of a Spinal Cord Injury Model Permissive to Study the Cardiovascular Effects of Rehabilitation Approaches Designed to Induce Neuroplasticity. Biology 2021, 10, 1006. https://doi.org/10.3390/biology10101006
Wainman L, Erskine EL, Ahmadian M, Hanna TM, West CR. Development of a Spinal Cord Injury Model Permissive to Study the Cardiovascular Effects of Rehabilitation Approaches Designed to Induce Neuroplasticity. Biology. 2021; 10(10):1006. https://doi.org/10.3390/biology10101006
Chicago/Turabian StyleWainman, Liisa, Erin L. Erskine, Mehdi Ahmadian, Thomas Matthew Hanna, and Christopher R. West. 2021. "Development of a Spinal Cord Injury Model Permissive to Study the Cardiovascular Effects of Rehabilitation Approaches Designed to Induce Neuroplasticity" Biology 10, no. 10: 1006. https://doi.org/10.3390/biology10101006