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Open AccessArticle

Antibacterial Activity of TiO2 Photocatalyst Alone or in Coatings on E. coli: The Influence of Methodological Aspects

Université de Toulouse, UPS, INSA, LMDC (Laboratoire Matériaux et Durabilité des Constructions), 135 Avenue de Rangueil, F-31077 Toulouse Cedex 04, France
Université de Toulouse, UPS, LGC (Laboratoire de Génie Chimique), Dép. BioSyM, UFR Pharmacie–35 rue des Maraîchers, 31062 Toulouse Cedex 09, France
Author to whom correspondence should be addressed.
Coatings 2014, 4(3), 670-686;
Received: 1 July 2014 / Revised: 4 August 2014 / Accepted: 12 August 2014 / Published: 22 August 2014
PDF [458 KB, uploaded 22 August 2014]


In damp environments, indoor building materials are among the main proliferation substrates for microorganisms. Photocatalytic coatings, including nanoparticles of TiO2, could be a way to prevent microbial proliferation or, at least, to significantly reduce the amount of microorganisms that grow on indoor building materials. Previous works involving TiO2 have already shown the inactivation of bacteria by the photocatalysis process. This paper studies the inactivation of Escherichia coli bacteria by photocatalysis involving TiO2 nanoparticles alone or in transparent coatings (varnishes) and investigates different parameters that significantly influence the antibacterial activity. The antibacterial activity of TiO2 was evaluated through two types of experiments under UV irradiation: (I) in slurry with physiological water (stirred suspension); and (II) in a drop deposited on a glass plate. The results confirmed the difference in antibacterial activity between simple drop-deposited inoculum and inoculum spread under a plastic film, which increased the probability of contact between TiO2 and bacteria (forced contact). In addition, the major effect of the nature of the suspension on the photocatalytic disinfection ability was highlighted. Experiments were also carried out at the surface of transparent coatings formulated using nanoparticles of TiO2. The results showed significant antibacterial activities after 2 h and 4 h and suggested that improving the formulation would increase efficiency. View Full-Text
Keywords: antibacterial activity; TiO2; photocatalysis; E. coli; transparent coating antibacterial activity; TiO2; photocatalysis; E. coli; transparent coating

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Verdier, T.; Coutand, M.; Bertron, A.; Roques, C. Antibacterial Activity of TiO2 Photocatalyst Alone or in Coatings on E. coli: The Influence of Methodological Aspects. Coatings 2014, 4, 670-686.

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