Comment on Celentano et al. Suitability of a Progenitor Cell-Enriching Device for In Vitro Applications. Coatings 2021, 11, 146
- A grid with hexagonal holes, each hole is equipped at the edges with micro-blades
- A rotating helix.
- As already mentioned, the authors claimed that the aim of the study was to “evaluate the potential of the device for an in vitro cell model”, even though the intended use of the device is to disaggregate mechanically tissue sample in a micrograft suspension.
- As reported in Sections 2.3 and 2.4 the authors processed a cellular suspension, already separated by trypsinization, with the Rigeneracons device.
- The Rigeneracons device allows us to disaggregate autologous human tissues (not cells), and is used with a specific amount of saline; both conditions were not respected in the study as the authors did not follow the manufactures guidelines/IFU (instructions for use). Consequently, the authors must state clearly that the device has been used without following the IFU to generate these “data”, which cannot be discussed regarding its relevance, nor can the clinical efficacy of the subjected medical device, as it has not been correctly used.
- The author claimed to separate progenitor cells from a population of fibroblasts, however it is mandatory to specify that the device is able to select the tissue cluster of a specific dimension containing progenitor cells due to hole size, as the device only act as a filter right after the tissue disaggregation. Again, it is difficult to understand the rationale perpetuated by the authors in using the Rigeneracons device only as a filter to collect progenitor cells; they could simply use a filter or a strainer. Even here, the scientific rationale in selecting progenitor cells from a single cell suspension using the Rigeneracons device is completely absent.
- A wide section of the article is written around the relevance of the metal debris found after the device’s use. Again, the device has not been used following the IFU, without any tissue sample, which generates more friction between the metal parts. There is no point in discussing/researching/publishing such information, as it is confusing and impolite toward the scientific community.
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Astarita, C.; Trovato, L.; Graziano, A. Comment on Celentano et al. Suitability of a Progenitor Cell-Enriching Device for In Vitro Applications. Coatings 2021, 11, 146. Coatings 2021, 11, 636. https://doi.org/10.3390/coatings11060636
Astarita C, Trovato L, Graziano A. Comment on Celentano et al. Suitability of a Progenitor Cell-Enriching Device for In Vitro Applications. Coatings 2021, 11, 146. Coatings. 2021; 11(6):636. https://doi.org/10.3390/coatings11060636
Chicago/Turabian StyleAstarita, Carlo, Letizia Trovato, and Antonio Graziano. 2021. "Comment on Celentano et al. Suitability of a Progenitor Cell-Enriching Device for In Vitro Applications. Coatings 2021, 11, 146" Coatings 11, no. 6: 636. https://doi.org/10.3390/coatings11060636
APA StyleAstarita, C., Trovato, L., & Graziano, A. (2021). Comment on Celentano et al. Suitability of a Progenitor Cell-Enriching Device for In Vitro Applications. Coatings 2021, 11, 146. Coatings, 11(6), 636. https://doi.org/10.3390/coatings11060636