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Open AccessFeature PaperArticle

Biocompatibility Evaluation and Enhancement of Elastomeric Coatings Made Using Table-Top Optical 3D Printer

1
Laser Research Center, Faculty of Physics, Vilnius University, Vilnius LT-10223, Lithuania
2
Life Sciences Center, Institute of Biochemistry, Vilnius University, Vilnius LT-10257, Lithuania
*
Author to whom correspondence should be addressed.
Coatings 2020, 10(3), 254; https://doi.org/10.3390/coatings10030254
Received: 3 January 2020 / Revised: 3 March 2020 / Accepted: 6 March 2020 / Published: 10 March 2020
(This article belongs to the Special Issue Thin Film Laser Damage, Ablation, Deposition and Structuring)
In this experimental report, the biocompatibility of elastomeric scaffold structures made via stereolithography employing table-top 3D printer Ember (Autodesk) and commercial resin FormLabs Flexible (FormLabs) was studied. The samples were manufactured using the standard printing and development protocol, which is known to inherit cytotoxicity due to remaining non-polymerized monomers, despite the polymerized material being fully biocompatible. Additional steps were taken to remedy this problem: the fabricated structures were soaked in isopropanol and methanol under different conditions (temperature and duration) to leach out the non-polymerized monomers. In addition, disc-shaped 3D-printed structures were UV exposed to assure maximum polymerization degree of the material. Post-processed structures were seeded with myogenic stem cells and the number of live cells was evaluated as an indicator for the material biocompatibility. The straightforward post-processing protocol enhanced the biocompatibility of the surfaces by seven times after seven days soaking in isopropanol and methanol and was comparable to control (glass and polystyrene) samples. This proposes the approach as a novel and simple method to be widely applicable for dramatic cytotoxicity reduction of optically 3D printed micro/nano-scaffolds for a wide range of biomedical studies and applications. View Full-Text
Keywords: stereolithography; elastomer; biocompatibility; post-processing; UV curing; thermal treatment; optical 3D printing stereolithography; elastomer; biocompatibility; post-processing; UV curing; thermal treatment; optical 3D printing
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Grigaleviciute, G.; Baltriukiene, D.; Bukelskiene, V.; Malinauskas, M. Biocompatibility Evaluation and Enhancement of Elastomeric Coatings Made Using Table-Top Optical 3D Printer. Coatings 2020, 10, 254.

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